Gonadotropins and cyclic adenosine 3',5'-monophosphate (cAMP) alter the morphology of cultured human granulosa cells.
Gonadotropins and cyclic adenosine 3',5'-monophosphate (cAMP) alter the morphology of cultured human granulosa cells.
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促性腺激素和环腺苷 3,5-单磷酸 (cAMP) 会改变培养的人颗粒细胞的形态。
DOI：
10.1095/biolreprod34.3.559
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发表时间：
1986
影响因子：
3.6
通讯作者：
Paavola,LG
中科院分区：
文献类型：
--
作者：
Soto,EA;Kliman,HJ;Strauss3rd,JF;Paavola,LG
应用相差显微镜、荧光显微镜、扫描电镜和透射电镜观察了体外培养的人卵巢颗粒细胞在加入人绒毛膜促性腺激素（hCG）、促黄体生成素（LH）、8-溴环腺苷酸（cAMP）和细胞松弛素（B、D）后的形态学变化。在这些药物的作用下，颗粒细胞的颗粒细胞变圆，形成指状突起附着在基质和相邻细胞上。细胞形状的变化伴随着线粒体和溶酶体向核周位置的向心运动。形态学的改变似乎是由环AMP介导的，是一个拆卸和重组的含microadhesive的应力纤维的结果。促卵泡激素（FSH）、催乳素（PRL）、生长激素（GH）和人胎盘催乳素（hPL）未引起细胞形状变化。我们的结论是，能够刺激促黄体化颗粒细胞分泌的促性腺激素引起细胞结构的变化，在体外，这导致参与类固醇合成的细胞器的重新分配。可能的关系的细胞骨架类固醇被认为是。
Morphological changes in human granulosa cells in culture were observed by phase, fluorescent, scanning electron and transmission electron microscopy following the addition of human chorionic gonadotropin (hCG), luteinizing hormone (LH), 8-bromocyclic adenosine 3′,5′-monophosphate (cAMP) and cytochalasins B and D. In response to these agents, polygon-shaped granulosa cells with granular cytoplasm became rounded, leaving fingerlike processes attached to the substratum and adjacent cells. The changes in cell shape were accompanied by a centripetal movement of mitochondria and lysosomes to a perinuclear location. The morphological alterations appeared to be mediated by cyclic AMP and to be the result of a dismantling and reorganization of microfilament-containing stress fibers. Follicle-stimulating hormone (FSH), prolactin (PRL), growth hormone (GH), and human placental lactogen (hPL) did not provoke cell shape changes. We conclude that tropic hormones capable of stimulating progestin secretion by luteinized granulosa cells cause a change in cell structure in vitro which leads to a redistribution of organelles involved in steroid synthesis. The possible relationship of the cytoskeleton to steroidogenesis is considered.