Biomimetic Microfluidic System for Fast and Specific Detection of Circulating Tumor Cells.
Biomimetic Microfluidic System for Fast and Specific Detection of Circulating Tumor Cells.
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DOI：
10.1021/acs.analchem.9b03920
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发表时间：
2019-11
影响因子：
7.4
通讯作者：
Fan Zhang;Lili Wu;Weidong Nie;Lili Huang;Jinfeng Zhang;Feng Li;Hai‐Yan Xie
中科院分区：
文献类型：
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作者：
Fan Zhang;Lili Wu;Weidong Nie;Lili Huang;Jinfeng Zhang;Feng Li;Hai‐Yan Xie
提高CTC的特异性捕获效率，同时防止周围背景细胞的非特异性吸附是CTC检测的主要研究方向。本文结合仿生纳米颗粒和微流控技术的独特优势，开发了一种新型的仿生微流控系统。磁性纳米团簇用白细胞膜片段伪装并用EpCAM阳性肿瘤细胞特异性适体SYL3C修饰，然后在磁体的帮助下加载到微流控芯片中。该系统在20 min内可捕获并检测出血液中90%以上的稀有肿瘤细胞，且几乎无白细胞背景，具有很大的实际应用潜力。
Improving the specific capture efficiency of CTCs, meanwhile preventing the nonspecific adsorption of surrounding back-ground cells is the main focus of CTCs detection. Herein, a novel biomimetic microfluidic system was developed by combin-ing the unique benefits of biomimetic nanoparticles and microfluidic techniques. The magnetic nanoclusters were camou-flaged with leukocyte membrane fragments and decorated with aptamer SYL3C specific for EpCAM positive tumor cells, and then loaded into the microfluidic chip with the help of magnets. By using of this system, more than 90% of the rare tu-mor cells in blood could be captured and detected within 20 min while almost no leukocyte background, indicating a great practical application potential for CTCs detection.