Fabrication of multilayer tubular scaffolds with aligned nanofibers to guide the growth of endothelial cells
Fabrication of multilayer tubular scaffolds with aligned nanofibers to guide the growth of endothelial cells
复制标题
制造具有排列纳米纤维的多层管状支架以引导内皮细胞的生长
DOI：
10.1177/0885328220935090
复制
发表时间：
2020-07-01
影响因子：
2.9
通讯作者：
Li, Shuai
中科院分区：
文献类型：
--
作者：
Hu, Qingxi;Su, Caiping;Li, Shuai
定向电纺纤维可调控细胞定向排列和相关功能表达，在组织工程中具有重要的应用价值。尽管在过去的十年中，小直径血管移植物（SDVG）的制造取得了重大进展，但仍存在一些挑战;其中最有问题的是用于多层SDVG的对齐纳米纤维的制造。此外，在多层结构的制造过程中，难以避免每层之间的分层。本研究介绍了一种新的制造方法的微小分层四层管状支架（FLTSs），包括一个内部层与高度纵向排列的纳米纤维，两个中间层组成的静电纺丝倾斜和圆周排列的纤维，和一个外部层包括随机纤维。这些FLTS用于模拟天然血管的结构和功能。本文以热塑性聚氨酯（TPU）/聚己内酯（PCL）/聚乙二醇（PEG）为原料，采用静电纺丝技术制备了具有完全无规纤维层（RLTS）的FLTS或管状支架。通过水接触角分析测试TPU/PCL/PEG管状支架的表面润湿性。特别是，与RLTS相比，FLTS表现出优异的力学性能，具有更高的周向和纵向拉伸性能。此外，人脐静脉内皮细胞（HUVECs）在FLTS上的高存活率表明管状支架与RLTS相比具有生物相容性。FLTSs的排列和随机的复合结构有利于促进HUVECs的生长，细胞在FLTSs上的粘附和增殖能力比RLTSs上有上级的提高。这些结果表明，所制备的FLTS具有应用于血管组织再生和临床动脉置换的潜力。
Aligned electrospun fibers used for the fabrication of tubular scaffolds possess the ability to regulate cellular alignment and relevant functional expression, with applications in tissue engineering. Despite significant progress in the fabrication of small-diameter vascular grafts (SDVGs) over the past decade, several challenges remain; one of the most problematic of these is the fabrication of aligned nanofibers for multilayer SDVGs. Furthermore, delamination between each layer is difficult to avoid during the fabrication of multilayer structures. This study introduces a new fabrication method for minute delamination four-layer tubular scaffolds (FLTSs) that consist of an interior layer with highly longitudinal aligned nanofibers, two middle layers composed of electrospun sloped and circumferentially aligned fibers, and an exterior layer comprising random fibers. These FLTSs are used to simulate the structures and functions of native blood vessels. Here, thermoplastic polyurethane (TPU)/polycaprolactone (PCL)/polyethylene glycol (PEG) were electrospun to fabricate FLTSs or tubular scaffolds with completely random fibers layer (RLTSs). The surface wettability of the TPU/PCL/PEG tubular scaffold was tested by water contact angle analysis. In particular, compared with RLTSs, FLTSs showed excellent mechanical properties, with higher circumferential and longitudinal tensile properties. Furthermore, the high viability of the human umbilical vein endothelial cells (HUVECs) on the FLTSs indicated the biocompatibility of the tubular scaffolds comparing to RLTSs. The aligned and random composite structure of the FLTSs are conducive to promoting the growth of HUVECs, and the cell adhesion and proliferation on these scaffolds was found to be superior to that on RLTSs. These results demonstrate that the fabricated FLTSs have the potential for application in vascular tissue regeneration and clinical arterial replacements.