Utilizing a polymerase chain reaction method for the detection of Toxocara canis and T-cati eggs in soil
Utilizing a polymerase chain reaction method for the detection of Toxocara canis and T-cati eggs in soil
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DOI：
10.1017/s0022149x07241872
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发表时间：
2007-03-01
影响因子：
1.6
通讯作者：
Mizgajska-Wiktor, H.
中科院分区：
文献类型：
--
作者：
Fogt-Wyrwas, R.;Jarosz, W.;Mizgajska-Wiktor, H.
聚合酶链反应（PCR）技术已被用于区分T犬和T。从土壤中分离出来的猫卵，以前通过显微镜观察鉴定过。该方法，使用特异性引物鉴定的两个弓首线虫物种，在现场和实验室进行了评估。当从40 g土壤样品中仅回收单个或大量卵时，获得了成功的结果。该方法是敏感的，允许独立的卵发育阶段的材料的分析，并可适用于从土壤中回收的其他种类的寄生虫。
A polymerase chain reaction (PCR) technique has been used for the differentiation of T canis and T. cati eggs isolated from soil and previously identified from microscopical observations. The method, using specific primers for the identification of the two Toxocara species, was assessed in both the field and laboratory. Successful results were obtained when only a single or large numbers of eggs were recovered from 40 g soil samples. The method is sensitive, allows analysis of material independent of the stage of egg development and can be adapted for the recovery of other species of parasites from soil.