Hydration, not silicone, modulates the effects of keratinocytes on fibroblasts
Hydration, not silicone, modulates the effects of keratinocytes on fibroblasts
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DOI：
10.1006/jsre.1995.1227
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发表时间：
1995-12-01
影响因子：
2.2
通讯作者：
Jee, SH
中科院分区：
文献类型：
--
作者：
Chang, CC;Kuo, YF;Jee, SH
局部硅胶凝胶或硅胶乳膏与封闭敷料已被证明是治疗和预防增生性疤痕和瘢痕疙瘩的有效方法，但如何触发这种作用仍不清楚。表皮的水合作用和/或释放的低分子量硅油的细胞效应被认为是可能的机制。为了进一步阐明其作用机制，我们采用体外角化细胞-成纤维细胞共培养模型来研究硅酮和水合作用对细胞的影响。在该模型中，可以模拟局部硅胶凝胶或乳膏的临床使用情况或闭塞敷料的水化作用情况。该模型由半透膜隔开的两个腔室组成，其中上腔室为完全分化的层状上皮，下腔室为中单层成纤维细胞。角质形成细胞仅从基侧滋养，而将其顶端表面浸泡在硅油、石蜡、汉克斯平衡盐溶液或介质（水化）中；或者它暴露在空气中（控制）。在水化处理组中，与对照组相比，以[H-3]胸腺嘧啶掺入量测量的成纤维细胞的增殖及其胶原蛋白、糖胺聚糖的产生明显受到抑制，但角化细胞暴露于硅油或石蜡中并不影响成纤维细胞的行为。结果表明，水合作用，而不是硅酮，调节体外角质形成细胞-成纤维细胞的相互作用。这可能是局部硅胶或闭塞敷料治疗可能影响肥厚性疤痕和瘢痕疙瘩发展的一种可能机制。(C) 1995学术出版社，Inc。
Topical silicone gel or silicone cream with occlusive dressing has proved to be an efficacious method for the treatment and prevention of hypertrophic scars and keloids, but how this action is triggered remains unknown. Hydration of the epidermis and/or the cellular effects of the released low-molecular-weight silicone oil have been suggested as possible mechanisms. In order to further elucidate the mechanism, we used an in vitro keratinocyte-fibroblast coculture model to investigate the cellular effects of silicone and hydration. In this model, the condition of clinical usage of topical silicone gel or cream or the condition of hydration exerted by occlusive dressing could be mimicked. The model consisted of two chambers separated by a semipermeable membrane, in which a fully differentiated stratified epithelium is present in the upper chamber and medium and monolayer fibroblasts are located in the lower chamber. The keratinocytes were nourished from the basal side only, while the apical surface was submerged in silicone oil, paraffin, Hanks' balanced salt solution, or medium (hydration); or it was exposed to air (control). In the hydration-treated group, the proliferation of fibroblasts measured as [H-3]thymidine incorporation and their collagen, glycosaminoglycan production was significantly inhibited when compared with the controls, but exposure of the keratinocyters to silicone oil or paraffin did not influence fibroblast behavior. The results suggest that hydration, not silicone, modulates the in vitro keratinocyte-fibroblast interaction. This may be one possible mechanism by which topical silicone or occlusive dressing treatment may affect the development of hypertrophic scars and keloids. (C) 1995 Academic Press, Inc.