Adipose-derived stem cells for tissue repair and regeneration: ten years of research and a literature review.
Adipose-derived stem cells for tissue repair and regeneration: ten years of research and a literature review.
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DOI：
10.1272/jnms.76.56
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发表时间：
2009-04
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影响因子：
--
通讯作者：
H. Mizuno
中科院分区：
文献类型：
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作者：
H. Mizuno
用于修复和再生各种组织和器官的基于干细胞的疗法提供了一种范式转变，可以为许多疾病提供替代治疗方案。虽然胚胎干细胞和诱导多能干细胞在理论上是非常有益的，但它们的使用受到细胞法规，伦理考虑和遗传操作的各种限制。另一方面，成人干细胞更容易获得，既不需要伦理也不需要免疫反应考虑，只要它们是自体组织来源即可。许多研究集中在从骨髓基质中分离的间充质干细胞，其已被证明在体外具有成脂、成骨、成软骨、成肌和成神经潜能。然而，骨髓采集对患者来说是极其痛苦的，并且获得的细胞数量很少。与骨髓来源的间充质干细胞相比，脂肪来源的干细胞同样能够分化为中胚层来源的细胞和组织。由于人体脂肪组织是普遍存在的，并且在局部麻醉下容易大量获得，患者不适感很小，因此它可能为间充质组织再生和工程提供干细胞的替代来源。基于我们以前的实验研究结果，本文重点介绍了脂肪干细胞的分子特性，分化潜力，伤口愈合的潜力，以及未来在细胞治疗和组织工程中的作用。
Stem cell based therapies for the repair and regeneration of various tissues and organs offer a paradigm shift that may provide alternative therapeutic solutions for a number of diseases. Although embryonic stem cells and induced pluripotent stem cells are theoretically highly beneficial, there are various limitations to their use imposed by cell regulations, ethical considerations, and genetic manipulation. Adult stem cells, on the other hand, are more easily available, with neither ethical nor immunoreactive considerations, as long as they are of autologous tissue origin. Much research has focused on mesenchymal stem cells isolated from bone marrow stroma which have been shown to possess adipogenic, osteogenic, chondrogenic, myogenic, and neurogenic potential in vitro. However bone marrow procurement is extremely painful for patients and yields low numbers of harvested cells. When compared with bone marrow-derived mesenchymal stem cells, adipose-derived stem cells are equally capable of differentiating into cells and tissues of mesodermal origin. Because human adipose tissue is ubiquitous and easily obtainable in large quantities under local anesthesia with little patient discomfort, it may provide an alternative source of stem cells for mesenchymal tissue regeneration and engineering. Based on our previous experimental findings, this review highlights the molecular characteristics, the potential for differentiation, the potential for wound healing, and the future role of adipose-derived stem cells in cell-based therapies and tissue engineering.