Four natriuretic peptides (ANP, BNP, VNP and CNP) coexist in the sturgeon: identification of BNP in fish lineage
Four natriuretic peptides (ANP, BNP, VNP and CNP) coexist in the sturgeon: identification of BNP in fish lineage
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DOI：
10.1677/jme.0.0320547
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发表时间：
2004-04-01
影响因子：
3.5
通讯作者：
Takei, Y
中科院分区：
文献类型：
--
作者：
Kawakoshi, A;Hyodo, S;Takei, Y
在四足动物和硬骨鱼类中，利钠肽（NIP）家族由心房、脑/心室和c型NIPS（分别为ANP、BNP/VNP和CNP）三个成员组成，而在板鳃鱼类中只有CNP。为了追踪NIP家族在早期脊椎动物进化中的分化过程，我们尝试在原始鳐鱼中检测NIPS （Acipenser transmontanus）。出乎意料的是，我们从这种软骨鱼的心脏和大脑中分离出了四种不同的NP cdna。从缺乏从分子内环延伸的c端“尾”序列判断，来自大脑的单个INIP是CNP。通过在其c端（ANP）和长而保守的c端尾部序列（VNP）存在酰胺信号来判断，三个心脏NIPS中的两个是ANP和VNP。第三种心脏NP极有可能是BNP，因为它具有BNP的所有特征，包括：(1)分子内环内存在双碱性氨基酸；(2)在其mRNA的t -非翻译区存在AUUUA重复序列；(3)其mRNA在心房和脑室中有相当的表达，在大脑中有可观的表达。基于鲟鱼BNP序列，我们在硬骨鱼类中首次从罗非鱼和河豚心脏中分离到BNP cDNA。前体系统发育分析表明，新鉴定的NIPS属于4个NIPS的每一个类群。目前对鲟鱼中VNP和BNP的鉴定清楚地表明，BNP和VNP是由不同的基因编码的，并且INIP家族在鳐鱼中至少有四个成员。VNP尚未在哺乳动物中进行分子鉴定，但从生理学研究中可以看出它的存在；异源鱼VNP在离体犬冠状动脉中表现出比同源哺乳动物ANP更强的血管松弛活性。
The natriuretic peptide (NIP) family is composed of three members: atrial, brain/ventricular and C-type NIPS (ANP, BNP/VNP and CNP respectively) in tetrapods and teleostean fish, but only CNP in elasmobranch fish. In order to trace the process of divergence of the NIP family in early vertebrate evolution, we attempted to detect NIPS in the primitive ray-finned fish, the sturgeon (Acipenser transmontanus). Unexpectedly, we isolated four distinct NP cDNAs from the heart and brain of this chondrostean fish. The single INIP from the brain was CNP, as judged from the lack of C-terminal 'tail' sequence extending from the intramolecular ring. Two of the three cardiac NIPS were ANP and VNP, as judged by the presence of an amidation signal at its C-terminus (ANP) and a long and conserved C-terminal tail sequence (VNP) respectively. The third cardiac NP was most probably BNP because it possessed all the features characteristic of BNP including: (1) the presence of dibasic amino acids within the intramolecular ring; (2) the presence of AUUUA repeats in the T-untranslated region of its mRNA; (3) equivalent expression of its mRNA in the atrium and ventricle and appreciable expression in the brain. Based on the sturgeon BNP sequence, we further isolated BNP cDNA from the heart of tilapia and pufferfish for the first time in teleostean fish. Phylogenetic analysis of the precursors showed that newly identified NIPS belong to each group of the four NIPS. The current identification of both VNP and BNP in the sturgeon clearly showed that BNP and VNP are coded by distinct genes, and that the INIP family consists of at least four members in the ray-finned fish. VNP has not been molecularly identified in mammals but its presence is suggested from physiological studies; heterologous fish VNP exhibited more potent vasorelaxant activity than homologous mammalian ANP in the isolated coronary artery of dogs.