Effect of human immunodeficiency virus‐1 protease inhibitors on the clearance of human herpesvirus 8 from blood of human Immunodeficiency virus‐1‐infected patients
Effect of human immunodeficiency virus‐1 protease inhibitors on the clearance of human herpesvirus 8 from blood of human Immunodeficiency virus‐1‐infected patients
复制标题
人类免疫缺陷病毒-1蛋白酶抑制剂对人类免疫缺陷病毒-1感染患者血液中人类疱疹病毒8型清除的影响
作者：
J. Leão;J. Leão;N. Kumar;K. McLean;S. Porter;C. Scully;A. Swan;C. Teo
评价了人类免疫缺陷病毒1型蛋白酶抑制剂对人类免疫缺陷病毒阳性患者外周血中人类疱疹病毒8型DNA检测频率的影响。对33例人类免疫缺陷病毒血清阳性男性患者进行了纵向研究。在引入蛋白酶抑制剂治疗之前和之后，通过聚合酶链反应从外周血的CD 45+部分扩增来自人疱疹病毒8型基因组的开放阅读框26的DNA。治疗前后还评估了人类疱疹病毒8 IgG状态、CD 4+细胞计数和人类免疫缺陷病毒-1血浆病毒载量。当同时引入逆转录酶抑制剂和蛋白酶抑制剂治疗时，CD 4 + T细胞计数增加（P=0.0041），人类免疫缺陷病毒血浆载量减少（P=0.0584），人类疱疹病毒8 DNA检出率降低（P=0.0077）。对已经接受逆转录酶抑制剂治疗的患者引入蛋白酶抑制剂与CD 4 + T细胞计数增加（P=0.0003）、人类免疫缺陷病毒血浆病毒载量降低（P=0.0911）和人类疱疹病毒8型检测率降低（P=0.0412）相关。未观察到抗人疱疹病毒8型IgG滴度的显著变化。因此，人类免疫缺陷病毒1型蛋白酶抑制剂治疗与人类免疫缺陷病毒感染患者外周血中人类疱疹病毒8型DNA的清除相关。伴随的人类免疫缺陷病毒血浆载量降低和外周血CD 4+细胞计数增加表明，人类免疫缺陷病毒1型感染负荷降低后免疫缺陷的改善是蛋白酶抑制剂清除人类疱疹病毒8的原因。医学病毒学杂志62：416-420，2000.© 2000 Wiley利斯公司
The effect of human immunodeficiency virus‐1 protease inhibitors on the frequency of human herpesvirus 8 DNA detection from peripheral blood of human immunodeficiency virus‐positive persons was evaluated. Thirty‐three human immunodeficiency virus‐seropositive male patients were studied longitudinally. DNA from open reading frame 26 of the human herpesvirus 8 genome was amplified by the polymerase chain reaction from the CD45+ fraction of peripheral blood before and after the introduction of protease inhibitor therapy. Human herpesvirus 8 IgG status, CD4+ cell counts, and human immunodeficiency virus‐1 plasma viral load were also assessed before and after therapy. When both reverse transcriptase inhibitor and protease inhibitor treatment were introduced at the same time, there was an increase in CD4+ T cell counts (P=0.0041), a decrease in human immunodeficiency virus plasma load (P=0.0584), and a decrease in the detection rate of human herpesvirus 8 DNA (P=0.0077). Introducing protease inhibitor to patients already receiving reverse transcriptase inhibitor treatment was associated with an increase in CD4+ T cell counts (P=0.0003), a decrease in human immunodeficiency virus plasma viral load (P=0.0911), and a decrease in the human herpesvirus 8 detection rate (P=0.0412). No significant changes in the titters of anti‐human herpesvirus 8 IgG were observed. Treatment with human immunodeficiency virus‐1 protease inhibitors is therefore associated with the clearance of human herpesvirus 8 DNA from peripheral blood of human immunodeficiency virus‐infected patients. The concomitant decrease in the human immunodeficiency virus plasma load and increase in the peripheral CD4+ cell count suggest that an amelioration in the immune defect following reduction in the burden of human immunodeficiency virus‐1 infection is responsible for the clearance of human herpesvirus 8 by protease inhibitors. J. Med. Virol. 62:416–420, 2000. © 2000 Wiley‐Liss, Inc.
影响因子：
56.9
作者：
WLODAWER, A;MILLER, M;KENT, SBH
通讯作者：
KENT, SBH