Acetylcholine release is elicited in the visual cortex, but not in the prefrontal cortex, by patterned visual stimulation:: A dual in vivo microdialysis study with functional correlates in the rat brain
Acetylcholine release is elicited in the visual cortex, but not in the prefrontal cortex, by patterned visual stimulation:: A dual in vivo microdialysis study with functional correlates in the rat brain
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DOI：
10.1016/j.neuroscience.2004.11.059
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发表时间：
2005-01-01
期刊：
影响因子：
3.3
通讯作者：
Vaucher, E
中科院分区：
文献类型：
--
作者：
Laplante, F;Morin, Y;Vaucher, E
通过对初级视觉和前额皮质的投射，基底前脑胆碱能系统参与感觉刺激的认知处理。有人提出，视觉刺激诱导的视觉皮层胆碱能激活可能对丘脑皮质输入发挥许可作用。然而，尚不清楚在没有注意力需要的情况下视觉刺激是否会引起高阶大脑区域的胆碱能激活。在本研究中，我们通过双探针体内微透析测量了图案化视觉刺激（水平光栅）对麻醉大鼠视觉和前额叶皮质中乙酰胆碱释放的影响。我们还使用逆行追踪来确定胆碱能神经元与视觉系统和前额皮质神经元的解剖关系。最后，我们评估了这种刺激的功能相关性，即 c-fos 免疫标记。图案化视觉刺激引起视觉皮层中乙酰胆碱释放的显着增加，同时该脑区域中 c-fos 免疫反应性神经元数量增加。相反，在前额皮质中，乙酰胆碱释放水平和 c-fos 免疫反应神经元数量都没有因刺激而发生显着变化。投射到视觉或前额皮质的胆碱能基底前脑神经元均位于布罗卡对角带的水平肢内，但在视觉刺激期间对 c-fos 没有免疫反应。没有发现视觉系统的任何部分直接投射到这些基底前脑神经元。这些结果表明胆碱能投射在感觉刺激整合中的不同参与，具体取决于目标皮质区域的活动水平。 (c) 2005 年国际广播组织。由爱思唯尔有限公司出版。保留所有权利。
By its projections to the primary visual and the prefrontal cortices, the basal forebrain cholinergic system is involved in cognitive processing of sensory stimuli. It has been suggested that visual stimulus-induced cholinergic activation of the visual cortex may exert a permissive role on thalamocortical inputs. However, it is not known if visual stimulation elicits cholinergic activation of high-order brain areas in the absence of attentional need. In the present study, we measured the effects of patterned visual stimulation (horizontal grating) on the release of acetylcholine with dual-probe in vivo microdialysis in the visual and the prefrontal cortices of anesthetized rats. We also used retrograde tracing to determine the anatomical relationships of cholinergic neurons with neurons of the visual system and the prefrontal cortex. Finally, we evaluated a functional correlate of this stimulation, namely c-fos immunolabeling. Patterned visual stimulation elicited significant increases in acetylcholine release in the visual cortex, accompanied by an increased number of c-fos immunoreactive neurons in this brain area. In contrast, in the prefrontal cortex, neither the level of acetylcholine release nor the number of c-fos immunoreactive neurons was significantly changed because of the stimulation. Cholinergic basal forebrain neurons projecting to the visual or the prefrontal cortices were both localized within the horizontal limb of the diagonal band of Broca but were not immunoreactive for c-fos during visual stimulation. No parts of the visual system were found to directly project to these basal forebrain neurons. These results suggest the differential involvement of cholinergic projections in the integration of sensory stimuli, depending on the level of activity of the targeted cortical area. (c) 2005 IBRO. Published by Elsevier Ltd. All rights reserved.