ASCITIC FLUID FROM HUMAN OVARIAN-CANCER PATIENTS CONTAINS GROWTH-FACTORS NECESSARY FOR INTRAPERITONEAL GROWTH OF HUMAN OVARIAN ADENOCARCINOMA CELLS
ASCITIC FLUID FROM HUMAN OVARIAN-CANCER PATIENTS CONTAINS GROWTH-FACTORS NECESSARY FOR INTRAPERITONEAL GROWTH OF HUMAN OVARIAN ADENOCARCINOMA CELLS
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DOI：
10.1172/jci114784
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发表时间：
1990-09-01
影响因子：
15.9
通讯作者：
MARKS, A
中科院分区：
文献类型：
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作者：
MILLS, GB;MAY, C;MARKS, A
卵巢癌是妇科恶性肿瘤死亡的主要原因，直到疾病晚期，卵巢癌往往仍局限于腹膜腔。在已确诊的疾病中，腹水在腹膜腔内积聚。我们之前已经证明，这种腹水是体外有丝分裂活性的有效来源，包括至少一种独特的生长因子。我们现在报道，在免疫缺陷的裸鼠体内，有卵巢癌患者腹水的存在(23/78只)，但不能诱导(0/21只)卵巢癌患者的腹水，人卵巢腺癌细胞株HY可以在裸鼠体内生长。良性疾病患者的腹水对HY细胞(1/15小鼠)的腹膜内生长没有类似的影响。一旦肿瘤是通过注射外源性腹水建立起来的，它们可以在没有额外注射腹水的情况下进展。小鼠最终产生了含有强大生长因子活性的腹水，这表明肿瘤最终产生了自体生长因子。这种裸鼠模型为研究卵巢癌生长因子在体内对肿瘤生长的作用提供了一个系统，并评估了临床前旨在抵消这些生长因子活性的治疗方法。
Human ovarian cancer, the leading cause of death from gynecologic malignancy, tends to remain localized to the peritoneal cavity until late in the disease. In established disease, ascitic fluid accumulates in the peritoneal cavity. We have previously demonstrated that this ascitic fluid is a potent source of in vitro mitogenic activity including at least one unique growth factor. We now report that the human ovarian adenocarcinoma line, HEY, can be induced to grow intraperitoneally in immunodeficient nude mice in the presence (23/78 mice), but not absence (0/21 mice) of ascitic fluid from ovarian cancer patients. Ascitic fluid from patients with benign disease did not have similar effects on intraperitoneal growth of HEY cells (1/15 mice). Once tumors were established by injections of exogenous ascitic fluid, they could progress in the absence of additional injections of ascitic fluid. The mice eventually developed ascitic fluid which contained potent growth factor activity, suggesting that the tumors eventually produced autologous growth factors. This nude mouse model provides a system to study the action of ovarian cancer growth factors on tumor growth in vivo and to evaluate preclinically, therapeutic approaches designed to counteract the activity of these growth factors.