Transcriptional Control of a Plant Stem Cell Niche
Transcriptional Control of a Plant Stem Cell Niche
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DOI：
10.1016/j.devcel.2010.03.012
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发表时间：
2010-05-18
影响因子：
11.8
通讯作者：
Lohmann, Jan U.
中科院分区：
文献类型：
--
作者：
Busch, Wolfgang;Miotk, Andrej;Lohmann, Jan U.
尽管植物和动物的多细胞性是独立进化的，但它们的干细胞生态位的基本组织非常相似。在这里，我们报告了WUSCHEL的全基因组调控潜力，WUSCHEL是参考植物拟南芥茎尖分生组织中干细胞维持的关键转录因子。WUSCHEL通过直接结合100多个靶启动子中的至少两个不同的DNA基序起作用，并优先影响在激素信号传导、代谢和发育中起作用的基因的表达。引人注目的例子是CLAVATA 1的直接转录抑制，这是WUSCHEL负反馈调节的一部分，以及TOPLESS家族转录抑制因子的直接调节，这涉及生长素信号传导。我们的研究结果揭示了维持一个动态的和必要的干细胞生态位所需的复杂的转录程序。
Despite the independent evolution of multicellularity in plants and animals, the basic organization of their stem cell niches is remarkably similar. Here, we report the genome-wide regulatory potential of WUSCHEL, the key transcription factor for stem cell maintenance in the shoot apical meristem of the reference plant Arabidopsis thaliana. WUSCHEL acts by directly binding to at least two distinct DNA motifs in more than 100 target promoters and preferentially affects the expression of genes with roles in hormone signaling, metabolism, and development. Striking examples are the direct transcriptional repression of CLAVATA1, which is part of a negative feedback regulation of WUSCHEL, and the immediate regulation of transcriptional repressors of the TOPLESS family, which are involved in auxin signaling. Our results shed light on the complex transcriptional programs required for the maintenance of a dynamic and essential stem cell niche.