Analysis of grape ESTs: global gene expression patterns in leaf and berry
Analysis of grape ESTs: global gene expression patterns in leaf and berry
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DOI：
10.1016/s0168-9452(00)00335-6
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发表时间：
2000-10-08
期刊：
影响因子：
5.2
通讯作者：
Henry, R
中科院分区：
文献类型：
--
作者：
Ablett, E;Seaton, G;Henry, R
对2479条葡萄属浆果组织EST和2438条叶片EST的分析表明，与已知葡萄属蛋白的EST匹配率为1%，与植物蛋白的匹配率为72%，与非植物蛋白的匹配率为11%，与植物蛋白的不匹配率为16%（P[N] > 0.5）。在叶和浆果文库中的冗余水平是相似的。只有12%的基因匹配的EST是共同的两个库表明在两个组织中表达的基因的显着差异。通过将与已知蛋白质（得分> 80）的主要BLAST匹配分类为功能类别来估计叶和浆果中具有预测细胞作用的转录物的丰度。36%的叶子转录物参与光合作用，而浆果中只有3%。这是一个高得多的比例涉及的转录本的功能仅限于专门的细胞，比被发现时，33个人类组织的转录本进行了比较，使用类似的方法，这表明植物细胞可能涉及其细胞机器在更大程度上专门的活动比动物细胞。相对增强的特定转录因子的表达，和基因参与防御，解毒，应激反应，蛋白水解，交通和信号转导，表明浆果组织积极参与响应环境刺激。(C)2000爱思唯尔科学爱尔兰有限公司保留所有权利。
Analysis of 2479 ESTs from Vitis vinifera berry tissue and 2438 from leaf revealed that 1% of the ESTs match to known Vitis proteins, 72% to plant proteins, 11% to non-plant, and 16% had no match (P[N] > 0.5). The levels of redundancy were similar in the leaf and berry libraries. Only 12% of the genes matched by the ESTs were common to both libraries indicating marked differences in the genes expressed in the two tissues. The abundance of transcripts with predicted cellular roles in leaf and berry were estimated by classifying the primary BLAST matches to known proteins (score > 80) into functional categories. Thirty-six percent of the leaf transcripts were involved in photosynthesis, compared to 3% in the berry. This is a much higher proportion of transcripts involved with a function limited to specialized cells, than was found when transcripts of 33 human tissues were compared using a similar approach, suggesting plant cells may involve their cellular machinery to a greater extent in specialized activities than animal cells. Relatively enhanced expression of specific transcription factors, and genes involved in defense, detoxification, stress response, proteolysis, trafficing, and signal transduction, suggests berry tissue is actively engaged in responding to environmental stimuli. (C) 2000 Elsevier Science Ireland Ltd. All rights reserved.