Suppression and overexpression of Lsi1 induce differential gene expression in rice under ultraviolet radiation
Suppression and overexpression of Lsi1 induce differential gene expression in rice under ultraviolet radiation
复制标题
紫外线照射下Lsi1的抑制和过表达诱导水稻差异基因表达
DOI：
10.1007/s10725-011-9569-y
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发表时间：
2011-09-01
影响因子：
4.2
通讯作者：
Lin, Wen-Xiong
中科院分区：
文献类型：
--
作者：
Fang, Chang-Xun;Wang, Qing-Shui;Lin, Wen-Xiong
低硅水稻基因 1 (Lsi1) 属于 Nod26 样主要内在蛋白 (NIP) 亚家族的成员，被认为控制水稻中硅 (Si) 的积累。为了进一步阐明其在水稻植物防御增强的紫外线B (UV-B)辐射胁迫中的调节机制，在耐UV-B水稻品种Lemont中对Lsi1进行抑制和过表达处理，并在对UV-B敏感的水稻品种Dular中进行过表达处理。结果表明，与野生型（WT）相比，在Lsi1过表达的水稻Lemont和Dular转基因品系中，Lsi1的转录水平增加，但在Lemont的Lsi1-RNAi转基因品系中，Lsi1的转录水平下调。根部硅吸收量和叶片硅浓度也有类似的趋势。此外，将不同的转基因水稻品系及其野生型暴露于增强的UV-B辐射下，通过抑制消减杂交（SSH）研究发现，Lsi1不仅可以调节苯丙氨酸氨裂解酶（PAL）、4-香豆酸辅酶A连接酶（4CL）和光裂解酶（PL）的表达，而且还可以诱导其他信号转导、解毒、抗性和光合作用相关基因。研究结果表明，通过增强/抑制 Lsi1 的表达来调节硅营养物质可以有效诱导与水稻耐受性相关的 mRNA 的转录。
Low silicon rice gene 1 (Lsi1) belongs to a member of Nod26-like major intrinsic protein (NIP) subfamily and is thought to control silicon (Si) accumulation in rice. In order to further elucidate its regulatory mechanisms in the defense of rice plants to enhanced ultraviolet-B (UV-B) radiation stress, Lsi1 was subjected to suppressed and overexpressed treatments in a UV-B tolerance rice accession Lemont as well as to overexpressed treatment in a UV-B sensitive rice accession Dular. The results showed that transcript levels of Lsi1 increased in Lsi1-overexpressed transgenic lines of rice accessions Lemont and Dular, but down-regulated in Lsi1-RNAi transgenic line of Lemont in comparison with their wild types (WT). A similar tendency was found in the root Si uptake and the Si concentrations in leaves. Further, the different transgenic rice lines and their WT were exposed to enhance UV-B radiation, study by suppression subtractive hybridization (SSH) found that Lsi1 not only could regulate the expression of phenylalanine ammonia-lyase (PAL), 4-coumarate-CoA ligase (4CL) and photolyase (PL), but also could induce other signal transduction-, detoxification-, resistance-, and photosynthesis-related genes. The findings suggested that the regulation of silicon nutrient by enhancing/inhibiting expression of Lsi1 could effectively induce the transcription of the mRNAs relative to tolerance in rice.