Catgut Implantation at Acupoint Reduces Immune Reaction in a Rat Model of Allergic Rhinitis
Catgut Implantation at Acupoint Reduces Immune Reaction in a Rat Model of Allergic Rhinitis
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穴位埋线可降低过敏性鼻炎大鼠模型的免疫反应
DOI：
10.1155/2018/7629239
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发表时间：
2018-01-01
影响因子：
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通讯作者：
Zhong, Zhendong
中科院分区：
文献类型：
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作者：
Yang, Shasha;Wu, Jing;Zhong, Zhendong
变应性鼻炎(AR)是一种由IgE介导的免疫反应，以Th2型免疫模式和肥大细胞激活为特征。针灸是一种传统的治疗方法，采用埋线的方法治疗变应性鼻炎。然而，穴位埋线(CIAA)免疫调节作用的确切机制仍不清楚，部分原因是缺乏合适的实验动物模型。我们建立并优化了卵清蛋白诱导的大鼠变态反应性炎症模型，其特征是IL-4、sIgE和SP水平升高，干扰素-γ水平降低。在本研究中，我们进一步利用这一模型来研究刺激迎祥、足三里穴位的免疫调节作用，并阐明了SP、sIgE、IL-4、干扰素-γ、TLR2和TLR4的调节机制。OVA激发建立AR模型后，随机分为对照组、模型组、假手术组、CIAA 1周组、CIAA 2周组和布地奈德鼻喷剂组。C_1组和C_2组分别取双侧迎祥、足三里。多项分析和量化分析表明，由于埋线对穴位的持续刺激，C2组的AR症状较C1组有所改善。结论：迎祥、足三里穴位CIAA能有效降低AR模型大鼠的过敏症状和炎症参数。因此，CIAA治疗有可能成为AR的一种替代治疗方式。
Allergic rhinitis (AR), an IgE-mediated response, is characterized by a Th2-type immunological pattern together with mast cells activation. Acupuncture, with the use of implanted catgut, is a traditional therapy that has been widely applied for the treatment of AR. However, the exact mechanism of the immunomodulatory effects of catgut implantation at acupoint (CIAA) remains unclear, in part due to the lack of a suitable laboratory animal model. We developed and optimized a rat model of ovalbumin- (OVA-) induced allergic inflammation, characterized by increased IL-4, sIgE, and SP and reciprocal decrease of IFN-γ. In the present study, we have further used this model to address the immunomodulatory effects of CIAA stimulation at Yingxiang (LI20) and Zusanli (ST36) acupoints and to elucidate the mechanisms involved in the regulation of SP, sIgE, IL-4, IFN-γ, TLR2, and TLR4. After AR model was established via OVA challenge, the rats were randomized as follows: control, model, sham-operated, 1-week CIAA (C1), 2-week CIAA (C2), and Budesonide nasal spray. The C1 and C2 groups were subjected to the bilateral acupoint Yingxiang (LI20) and Zusanli (ST36), respectively. Multiple analyses and quantifications were performed, which revealed that due to the persistent stimulus to acupoints by embedding catgut, the C2 group improved AR symptoms, compared to the C1 group. We conclude that CIAA at the Yingxiang (LI20) and Zusanli (ST36) acupoints effectively reduces allergic symptoms and inflammatory parameters in the rat model of AR. Thus, CIAA treatment is potentially an alternative therapeutic modality in AR.