BIOCHEMICAL RESISTANCE DETECTION - AN ALTERNATIVE TO BIOASSAY
BIOCHEMICAL RESISTANCE DETECTION - AN ALTERNATIVE TO BIOASSAY
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DOI：
10.1016/0169-4758(89)90192-0
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发表时间：
1989-02-01
期刊：
影响因子：
--
通讯作者：
BROGDON, WG
中科院分区：
文献类型：
--
作者：
BROGDON, WG
Insecticide resistance is an increasing problem in vector control programrnes. Until resistance detection assays have been or recently, the usual means of detecting it has been by bioassay, requiring the use of are being developed. relatively large numbers of insects and insecticide-impregnated test papers which may be These new methods feature detec-di~ cult to prepare and store reproducibly. William Brogdon argues for the use of tion of resistance and determination of biochemical microplate assays which are cheaper and easier to use, permit up to 30 its biochemical mechanisms in single assays to be made on a single insect, and give more reproducible results, insects in the field, and the biochemical assays are both cheaper and simpler Insecticide resistance has continually resistance detection technology available than conventional bioassays. Most challenged entomologists who seek for use in the field, it is far from perfect important to field workers, resistance means to retard its development or (Box 2). As a result, biochemical, can be detected at very low frequency, soften its impact on financially limited immunological, and nucleic acid probe allowing efficient monitoring of vector control programmes~. Resistance has been reported in hundreds of insect species, but its recognition has usually resulted from control failure, seldom if Box I. Resistance Mechanisms and Cross-resistance Spectra ever with early warning 2. Conventional Microplate detection methods exist for all the resistance mechanisms shown below except knockdown resistance (kdr). The mechanisms shown are all biochemical; detection of resistance is based on behavioural resistance is not detected by bioa~ ay or microassay. Theoretically, one insecticide susceptibility tests which are microassay method (target enzyme method) would detect the reduced penetration dosage-mortality (bioassay) experi- resistance mechanism, but experimental evidence is lacking. ments, not designed to detect underlying 3 resistance mechanisms. Although the
