A set of SNPs near or within STR regions useful for discriminating native Lake Biwa and introduced "Eurasian" strains of common carp
A set of SNPs near or within STR regions useful for discriminating native Lake Biwa and introduced "Eurasian" strains of common carp
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DOI：
10.1007/s12686-012-9613-x
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发表时间：
2012-09-01
影响因子：
1.1
通讯作者：
Nishida, Mutsumi
中科院分区：
文献类型：
--
作者：
Mabuchi, Kohji;Song, Ha Yeun;Nishida, Mutsumi
我们提出了一套核DNA SNPs用于区分本地的琵琶湖和引进的“欧亚”株普通鲤鱼，并进一步检测它们的杂交。在短串联重复（STR）区域周围搜索这样的SNP，以稍后用作HapSTR标记。采用PCR和直接测序的方法共筛选出62个微卫星位点。从典型的琵琶湖（n = 30）和“欧亚”（n = 24）标本中得到的核苷酸序列表明，分布在7个位点上的总共21个SNP将用于上述目的。所有的SNP都可以通过直接测序进行基因分型，同时STR多态性。
We present a set of nuclear DNA SNPs useful for discriminating native Lake Biwa and introduced "Eurasian" strains of common carp, and further, for detecting their hybridization. Such SNPs were searched around short tandem repeat (STR) regions to be used later as HapSTR markers. A total of 62 microsatellite locus were screened with PCR and direct sequencing. The resultant nucleotide sequences from typical Lake Biwa (n = 30) and "Eurasian" (n = 24) specimens indicated that a total of 21 SNPs distributed across 7 loci would be useful for the above-mentioned purpose. All of the SNPs can be genotyped by direct sequencing, simultaneously with STR polymorphisms.