Analysis of loss of adhesive function in sperm lacking cyritestin or fertilin β
Analysis of loss of adhesive function in sperm lacking cyritestin or fertilin β
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DOI：
10.1006/dbio.2001.0166
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发表时间：
2001-05-01
影响因子：
2.7
通讯作者：
Primakoff, P
中科院分区：
文献类型：
--
作者：
Nishimura, H;Cho, C;Primakoff, P
我们制造了缺乏精子表面蛋白cryitestin （adam3）的小鼠，发现突变的雄性不育。与无受精β (adam2)精子类似（C. Cho等人，1998,Science 281, 1857-1859），无cyritestin精子对卵细胞透明带（野生型的0.3%）和卵细胞质膜（野生型的9%）的粘附能力严重不足。因此，缺失ADAM基因的雄性小鼠的精子在受精的至少两个步骤中失去了粘附功能。我们发现任何一个ADAM基因的缺失都会导致多个基因产物的丢失。这种多基因产物（精子膜蛋白）的丢失似乎是精子分化过程中一种新的发育机制造成的。由于精子蛋白表达的改变一定是导致受精缺陷的原因，我们的数据为受精过程中受影响步骤的分子基础提供了新的模型。(C) 2001学术出版社。
We produced mice lacking the sperm surface protein cryitestin (ADAM 3) and found mutant males are infertile. Similar to fertilin beta (ADAM 2) null sperm (C. Cho ef al., 1998, Science 281, 1857-1859), cyritestin null sperm are drastically deficient in adhesion to the egg zona pellucida (0.3% of wild type) and to the egg plasma membrane (9% of wild type). Thus sperm from male mice with a gene deletion of either ADAM have a loss of adhesive function in at least two steps of fertilization. We found deletion of either ADAM gene resulted in the loss of multiple gene products. This loss of multiple gene products (sperm membrane proteins) appears to result from a novel, developmental mechanism during sperm differentiation. Because the altered sperm protein expression must be responsible for the fertilization defects, our data suggest new models for the molecular basis of the affected steps in fertilization. (C) 2001 Academic Press.