Effective Spatial Separation of PC12 and NIH3T3 Cells by the Microgrooved Surface of Biocompatible Polymer Substrates.
Effective Spatial Separation of PC12 and NIH3T3 Cells by the Microgrooved Surface of Biocompatible Polymer Substrates.
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通过生物相容性聚合物基底的微槽表面有效空间分离 PC12 和 NIH3T3 细胞
DOI：
10.1021/acs.langmuir.5b01018
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发表时间：
2015-06-23
期刊：
影响因子：
--
通讯作者：
Wang Y
中科院分区：
文献类型：
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作者：
Gao H;Dong H;Cao X;Fu X;Zhu Y;Mao C;Wang Y
大多数器官和组织由一种以上类型的细胞组成，这些细胞在空间上是分开的，并且位于不同的区域。本研究使用微槽聚(乳酸-乙醇酸)(PLGA)底物将两种类型的共培养细胞引导到两个空间分离的区域。具体地说，PC12嗜铬细胞瘤细胞被引导到微沟内侧，而NIH3T3成纤维细胞被引导到相邻平行微沟之间的脊区。此外，微槽结构还能显著促进PC12细胞的增殖和神经分化，以及NIH3T3细胞的成骨分化。因此，含有PC12和NIH3T3细胞的微槽PLGA表面可以作为研究骨修复支架内神经重建的潜在模型系统。
Most organs and tissues are composed of more than one type of cell that is spatially separated and located in different regions. This study used a microgrooved poly(lactic-co-glycolic acid) (PLGA) substrate to guide two types of cocultured cells to two spatially separated regions. Specifically, PC12 pheochromocytoma cells are guided to the inside of microgrooves, whereas NIH3T3 fibroblasts are guided to the ridge area in between neighboring parallel microgrooves. In addition, the microgrooved structures can significantly promote the proliferation and neural differentiation of PC12 cells as well as the osteogenic differentiation of NIH3T3 cells. Therefore, the microgrooved PLGA surface with separated PC12 and NIH3T3 cells can serve as a potential model system for studying nerve reconstruction in bone-repairing scaffolds.