Bidirectional Interplay between Vimentin Intermediate Filaments and Contractile Actin Stress Fibers
Bidirectional Interplay between Vimentin Intermediate Filaments and Contractile Actin Stress Fibers
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DOI：
10.1016/j.celrep.2015.05.008
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发表时间：
2015-06-16
期刊：
影响因子：
8.8
通讯作者：
Lappalainen, Pekka
中科院分区：
文献类型：
--
作者：
Jiu, Yaming;Lehtimaki, Jaakko;Lappalainen, Pekka
肌动蛋白细胞骨架和细胞质中间丝有助于细胞迁移和形态发生，但这两个中心细胞骨架元素之间的相互作用仍然难以捉摸。在这里，我们发现，特定的肌动蛋白应力纤维结构，横向弧，与波形蛋白中间丝相互作用，并促进其逆行流动。因此，肌球蛋白-II的弧是重要的波形蛋白网络在细胞核周围的本地化。波形蛋白网络限制弧的逆行运动，从而控制细胞前缘的扁平板层的宽度。plectin的耗尽概括了弧缺陷细胞的波形蛋白组织表型，而不影响波形蛋白丝或应力纤维的完整性，表明这种细胞骨架交联剂是波形蛋白和弧之间的生产性相互作用所需的。总的来说，我们的研究结果表明，plectin介导的收缩肌动球蛋白弧和波形蛋白中间丝之间的相互作用控制这两个细胞骨架系统的本地化和动态，因此是重要的细胞形态发生。
The actin cytoskeleton and cytoplasmic intermediate filaments contribute to cell migration and morphogenesis, but the interplay between these two central cytoskeletal elements has remained elusive. Here, we find that specific actin stress fiber structures, transverse arcs, interact with vimentin intermediate filaments and promote their retrograde flow. Consequently, myosin-II-containing arcs are important for perinuclear localization of the vimentin network in cells. The vimentin network reciprocally restricts retrograde movement of arcs and hence controls the width of flat lamellum at the leading edge of the cell. Depletion of plectin recapitulates the vimentin organization phenotype of arc-deficient cells without affecting the integrity of vimentin filaments or stress fibers, demonstrating that this cytoskeletal crosslinker is required for productive interactions between vimentin and arcs. Collectively, our results reveal that plectin-mediated interplay between contractile actomyosin arcs and vimentin intermediate filaments controls the localization and dynamics of these two cytoskeletal systems and is consequently important for cell morphogenesis.