High correlation between lipid peroxide radical and tumor-promoter effect: suppression of tumor promotion in the Epstein-Barr virus/B-lymphocyte system and scavenging of alkyl peroxide radicals by various vegetable extracts.
High correlation between lipid peroxide radical and tumor-promoter effect: suppression of tumor promotion in the Epstein-Barr virus/B-lymphocyte system and scavenging of alkyl peroxide radicals by various vegetable extracts.
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DOI：
10.1111/j.1349-7006.1992.tb02001.x
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发表时间：
1992-09
期刊：
影响因子：
--
通讯作者：
Yasutake R
中科院分区：
文献类型：
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作者：
Maeda H;Katsuki T;Akaike T;Yasutake R
我们检测了 66 种蔬菜和植物的热水提取物抑制肿瘤生长以及体外清除脂质过氧化自由基的能力。为了评估体外抗肿瘤促进（转化）的效果，我们使用了佛波醇肉豆蔻酸酯乙酸酯/Epstein-Barr病毒/B淋巴细胞系统。为了评估脂质自由基清除效果，使用了使用叔丁基过氧化氢/血红素系统的鲁米诺增强化学发光方法，该方法产生的烷基过氧化物自由基（ROO·）比烷基（R·）和烷氧基<RO）自由基更多。结果显示抗肿瘤促进作用与脂质自由基清除作用之间存在显着相关性（r=0.82）。我们发现胡萝卜、十字花科植物和豆类（黑豆、红豆、绿豆和大豆）绿叶的煮沸提取物具有最大的抗肿瘤促进剂和自由基清除活性。蔬菜的冷水提取物通常仅表现出热水提取物的约 10% 或更少的活性。
We examined the ability of hot‐water extracts of 66 vegetables and plants to suppress tumor promotion, as well as to scavenge lipid peroxide radicals in vitro. To assess the effect against tumor promotion (transformation) in vitro, we used the phorbol myristate acetate/Epstein‐Barr virus/ B‐lymphocyte system. To assess the lipid radical‐scavenging effect, the luminol‐enhanced cliemi‐luminescence method using the tot‐butyl hydroperoxide/heme system was used, which generates more alkyl peroxide radical (ROO·) than alkyl (R·) and alkoxyl <RO) radicals. The results showed a significant correlation between the anti‐tumor‐promoting effect and the lipid radical‐scavenging effect (r=0.82). We found that boiled extracts of green leaves of carrot, crucifers, and beans (black bean, red bean, mung bean, and soybean) had the greatest anti‐tumor‐promoter and radical‐scavenging activities. Cold‐water extracts of vegetables generally exhibited only about 10% or less of the activity of the hot‐water extracts.