Identification of Arabidopsis MYB56 as a Novel Substrate for CRL3BPM E3 Ligases
Identification of Arabidopsis MYB56 as a Novel Substrate for CRL3BPM E3 Ligases
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DOI：
10.1016/j.molp.2014.10.004
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发表时间：
2015-02-02
期刊：
影响因子：
27.5
通讯作者：
Hellmann, Hanjo
中科院分区：
文献类型：
--
作者：
Chen, Liyuan;Bernhardt, Anne;Hellmann, Hanjo
控制蛋白质的稳定性是指导活细胞中各种过程的高效机制。泛素蛋白酶体途径是蛋白质稳定性的一个关键调控系统，它使用E3连接酶来标记特定的蛋白质进行降解。在这项工作中，MYB56被鉴定为CULLIN3 （CUL3）为基础的E3连接酶的新靶点。它的稳定性取决于MATH-BTB/POZ （BPM）蛋白的存在，该蛋白作为E3连接酶的底物接头起作用。遗传研究表明，MYB56是开花的负调控因子，而bpm则积极影响这一发育程序。bpm与MYB56的相互作用发生在开花位点T（开花位点T是拟南芥开花启动的关键调控因子）的启动子上，并导致MYB56的不稳定性。总的来说，这项工作确定了MYB转录因子作为BPM蛋白的底物，并提供了参与控制植物开花时间的成分的新信息。
Controlled stability of proteins is a highly efficient mechanism to direct diverse processes in living cells. A key regulatory system for protein stability is given by the ubiquitin proteasome pathway, which uses E3 ligases to mark specific proteins for degradation. In this work, MYB56 is identified as a novel target of a CULLIN3 (CUL3)-based E3 ligase. Its stability depends on the presence of MATH-BTB/POZ (BPM) proteins, which function as substrate adaptors to the E3 ligase. Genetic studies have indicated that MYB56 is a negative regulator of flowering, while BPMs positively affect this developmental program. The interaction between BPMs and MYB56 occurs at the promoter of FLOWERING LOCUS T (FT), a key regulator in initiating flowering in Arabidopsis, and results in instability of MYB56. Overall the work establishes MYB transcription factors as substrates of BPM proteins, and provides novel information on components that participate in controlling flowering time in plants.