Expression of integrin-linked kinase is closely correlated with invasion and metastasis of gastric carcinoma
Expression of integrin-linked kinase is closely correlated with invasion and metastasis of gastric carcinoma
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DOI：
10.1007/s00428-002-0718-6
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发表时间：
2003-02-01
期刊：
影响因子：
3.5
通讯作者：
Yasui, W
中科院分区：
文献类型：
--
作者：
Ito, R;Oue, N;Yasui, W
整合素连接激酶（ILK）是一种丝氨酸/苏氨酸蛋白激酶，涉及细胞-基质相互作用和细胞增殖的细胞控制，与某些肿瘤的高度侵袭性表型相关。为探讨ILK是否参与胃癌的发生、发展，我们采用逆转录聚合酶链反应（RT-PCR）和免疫组化方法检测了ILK在胃癌细胞系、原发性胃癌及相应非肿瘤黏膜中的表达，并分析其与临床病理参数的关系。RT-PCR检测5株胃癌细胞系中有4株ILK mRNA表达，35例胃癌组织标本中有22例（63%）ILK mRNA表达。ILK mRNA在硬化型和中间型中的表达率（82%）明显高于髓样型（44%）（P=0.0204）。ILK mRNA表达与淋巴结转移密切相关（P=0.0388）。免疫组化结果显示，ILK蛋白在69%（84/122）的原发性胃癌中呈强阳性表达（阳性细胞率超过50%），而在非肿瘤性胃上皮中未见表达。ILK蛋白强表达与硬化型、中间型（P=0.0217）、胃壁浸润深度（P=0.0006）和淋巴结转移（P=0.0176）相关。这些结果提示，ILK不仅参与了胃癌尤其是硬化型和中间型胃癌的发生，而且还参与了胃癌的侵袭和转移。ILK可能是一种新的侵袭性胃癌的分子标志物。
Integrin-linked kinase (ILK), a serine/threonine protein kinase, implicates in cellular control of cell-matrix interactions and cell proliferation that is associated with a highly invasive phenotype of certain tumors. To study whether ILK is involved in the development and progression of gastric carcinoma, we examined the expression of ILK in gastric carcinoma cell lines, primary gastric carcinomas and corresponding non-neoplastic mucosa using reverse-transcription polymerase chain reaction (RT-PCR) and immunohistochemistry and analyzed the relationship with clinicopathological parameters. The expression of ILK mRNA was detected in 4 of 5 gastric carcinoma cell lines and 22 of 35 (63%) micro-dissected tumor samples of primary gastric carcinoma using RT-PCR. The incidence of cases with ILK mRNA expression was significantly higher in scirrhous and intermediate type (82%) than in medullary type (44%) (P=0.0204). Significant association was detected between ILK mRNA expression and presence of nodal metastasis (P=0.0388). Immunohistochemically, strong expression of ILK protein (over 50% of tumor cells were positive) was detected in 69% (84/122) of primary gastric carcinomas, whereas no ILK expression was found in non-neoplastic gastric epithelia. Strong expression of ILK protein was significantly associated with scirrhous and intermediate type (P=0.0217), deep invasion of tumor cells in gastric wall (P=0.0006), and presence of nodal metastasis (P=0.0176). These results strongly suggest that ILK may participate not only in stomach carcinogenesis especially of scirrhous and intermediate types but also in invasion and metastasis of gastric carcinoma. ILK might be a novel molecular marker for aggressive gastric cancer.