Nanocellulose aerogel inserts for quantitative lateral flow immunoassays
Nanocellulose aerogel inserts for quantitative lateral flow immunoassays
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DOI：
10.1016/j.bios.2021.113491
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发表时间：
2021-07-14
影响因子：
12.6
通讯作者：
Generelli, Silvia
中科院分区：
文献类型：
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作者：
Tang, Ye;Gao, Hui;Generelli, Silvia
侧向流动免疫分析(LFIA)是一种成熟的技术，无需昂贵的实验室设备和用户的技术技能就能立即产生结果。然而，传统的比色LFIA纸条检测下限较高，主要是由于分析的样品体积有限，目标分析物与偶联分子之间的反应时间较短，以及光学信号较弱。因此，LFIA主要被用作医学诊断工具，分别用于定性和半定量检测。我们将一种新型的纤维素纳米纤维(CNF)气凝胶材料加入到LFIA试条中，以增加样品流动时间，这反过来又延长了目标分析物与检测抗体之间的结合作用，从而提高了检测限(LOD)。与传统的LFIA纸条相比，气凝胶修饰的LFIA纸条中的样品流动时间更长，将缓冲溶液中小鼠免疫球蛋白检测的LOD提高了1000倍。建立的CNF气凝胶辅助LFIA法检测人血清中的免疫球蛋白，检测灵敏度为0.72 ng/mL。在改进的LOD基础上，气凝胶辅助LFIA可以定量测定缓冲液和人血清中的IgG样品，浓度范围分别为0.17 ng/mL~100 ng/mL(缓冲液)和4.6 ng/mL~100 ng/mL(人血清)。因此，提出的解决方案提供了独特的潜力，将侧向血流分析转变为高灵敏度、完全定量的护理点诊断。
The Lateral Flow Immuno Assay (LFIA) is a well-established technique that provides immediate results without high-cost laboratory equipment and technical skills from the users. However, conventional colorimetric LFIA strips suffer from high limits of detection, mainly due to the analysis of a limited sample volume, short reaction time between the target analyte and the conjugation molecules, and a weak optical signal. Thus, LFIAs are mainly employed as a medical diagnostic tool for qualitative and semi/quantitative detection, respectively. We applied a novel cellulose nanofiber (CNF) aerogel material incorporated into LFIA strips to increase the sample flow time, which in turn extends the binding interactions between the analyte of interest and the detection antibody, thus improving the limit of detection (LOD). Compared to a conventional LFIA strip, the longer sample flow time in the aerogel modified LFIA strips improved the LOD for the detection of mouse IgG in a buffer solution by a 1000fold. The accomplished LOD (0.01 ng/mL) even outperformed specifications of a commercial ELISA kit by a factor of 10, and the CNF aerogel assisted LFIA was successfully applied to detect IgG in human serum with a LOD of 0.72 ng/mL. Next to the improved LOD, the aerogel assisted LFIA could quantify IgG samples in buffer and human serum in the concentration ranges of 0.17 ng/mL - 100 ng/mL (in buffer) and 4.6 ng/mL - 100 ng/mL (in human serum). The presented solution thus poses a unique potential to transform lateral flow assays into highly sensitive, fully quantitative point-of-care diagnostics.