3D intensity and phase imaging from light field measurements in an LED array microscope
3D intensity and phase imaging from light field measurements in an LED array microscope
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DOI：
10.1364/optica.2.000104
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发表时间：
2015-02-20
期刊：
影响因子：
10.4
通讯作者：
Waller, Laura
中科院分区：
文献类型：
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作者：
Tian, Lei;Waller, Laura
对于具有高速采集的3D成像技术来说，在大体积上实现高分辨率是一个挑战。在这里，我们描述了一种从4D光场测量中恢复3D强度和相位的新方法，通过傅立叶重叠关联成像实现增强的分辨率。从几何光学的光场重聚焦出发，结合相位恢复和衍射伪影校正。此外，我们将暗场图像，以实现横向分辨率超过物镜的衍射极限（5倍大NA）和轴向分辨率优于景深，使用低放大率物镜与大视场。我们的迭代重建算法使用多层相干模型来估计样品在多个深度处的3D复透射率函数，而没有任何弱或单散射近似。数据由具有计算照明的LED阵列显微镜捕获，这使得能够快速扫描角度以进行快速采集。我们证明了厚的生物样品在一个修改后的商业显微镜的方法，表明该技术的多功能性广泛的应用。(C)2015美国光学学会
Realizing high resolution across large volumes is challenging for 3D imaging techniques with high-speed acquisition. Here, we describe a new method for 3D intensity and phase recovery from 4D light field measurements, achieving enhanced resolution via Fourier ptychography. Starting from geometric optics light field refocusing, we incorporate phase retrieval and correct diffraction artifacts. Further, we incorporate dark-field images to achieve lateral resolution beyond the diffraction limit of the objective (5x larger NA) and axial resolution better than the depth of field, using a low-magnification objective with a large field of view. Our iterative reconstruction algorithm uses a multislice coherent model to estimate the 3D complex transmittance function of the sample at multiple depths, without any weak or single-scattering approximations. Data are captured by an LED array microscope with computational illumination, which enables rapid scanning of angles for fast acquisition. We demonstrate the method with thick biological samples in a modified commercial microscope, indicating the technique's versatility for a wide range of applications. (C) 2015 Optical Society of America