Enhancing hypericin production of Hypericum perforatum cell suspension culture by ozone exposure
Enhancing hypericin production of Hypericum perforatum cell suspension culture by ozone exposure
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臭氧暴露提高贯叶金丝桃细胞悬浮培养物金丝桃素的产量
DOI：
10.1002/btpr.614
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发表时间：
2011
期刊：
影响因子：
--
通讯作者：
Xiangbin Xu
中科院分区：
文献类型：
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作者：
Maojun Xu;Bin Yang;Jufang Dong;Dan Lu;Haihong Jin;Lina Sun;Yun Zhu;Xiangbin Xu
次生代谢产物的积累是自然界中植物对臭氧暴露的常见反应之一。为了研究臭氧对植物细胞培养物中所需化合物产生的影响，我们测定了不同剂量臭氧处理的贯叶连翘悬浮细胞培养物在不同培养阶段金丝桃素的产生。结果表明，用60至180 nL L−1臭氧处理的细胞的金丝桃素含量显著高于对照组，表明臭氧暴露可能刺激金丝桃素合成。在指数期用臭氧处理的细胞的金丝桃素产量高于停滞期和稳定期，这表明指数期细胞培养物比停滞期和稳定期细胞对臭氧暴露更敏感。最高的金丝桃素产量是通过在指数期晚期暴露于90 nL L-1臭氧3 h获得的，约为对照的四倍。© 2011美国化学工程师学会生物技术。程序：2011
Accumulation of secondary metabolites is one of the common reactions of plants to ozone exposure in nature. To investigate the effect of ozone on the production of desired compounds of plant cell cultures, we assayed hypericin production of Hypericum perforatum suspension cell cultures treated with different doses of ozone at different culture phases. The results show that hypericin contents of the cells treated with 60 to 180 nL L−1 ozone are significantly higher than those of the control, showing that ozone exposure may stimulate hypericin synthesis. Hypericin production of the cells treated with ozone at exponential phase is higher than that of lag and stationary phase, which suggests that exponential phase cell cultures are more responsive to ozone exposure than lag and stationary phase cells. The highest hypericin production is obtained by the cells exposed to 90 nL L−1 ozone at late exponential phase for 3 h, being about fourfold of the control. © 2011 American Institute of Chemical Engineers Biotechnol. Prog., 2011