Changes in enteric neural regulation of smooth muscle in a rabbit model of small intestinal inflammation.
Changes in enteric neural regulation of smooth muscle in a rabbit model of small intestinal inflammation.
复制标题
兔小肠炎症模型中平滑肌肠神经调节的变化。
DOI：
10.1152/ajpgi.1995.268.5.g823
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
Shea-Donohue,T
中科院分区：
文献类型：
--
作者：
Goldhill,JM;Sanders,KM;Sjogren,R;Shea-Donohue,T
在体外电生理研究的兔与蓖麻毒素诱导的炎症回肠环形肌进行调查是否改变神经控制或生肌活性有助于先前描述的变化在体内肌电活动。蓖麻毒素治疗增加平均慢波振幅，但不频率或静息膜电位。长时间的电场刺激诱发的超极化过程中的刺激列车和去极化停止刺激。在阿托品的存在下，蓖麻毒素处理的组织比对照组织的去极化更大，表明回肠炎增强了非胆碱能兴奋。一氧化氮合酶抑制剂N-硝基-L-精氨酸甲酯降低了蓖麻毒素处理的超极化，但在对照组织中没有，表明炎症增加了一氧化氮介导的抑制。P物质脱敏减少非胆碱能兴奋和平均慢波振幅仅在蓖麻毒素处理的组织，表明这些参数的变化在炎症过程中导致增加释放，或敏感性，速激肽。这些数据表明，急性回肠炎改变速激肽和一氧化氮介导的神经传递，可能会影响回肠运动和/或感觉反射的正常模式。
In vitro electrophysiological studies of ileal circular muscle from rabbits with ricin-induced inflammation were performed to investigate whether altered neural control or myogenic activity contributes to previously described changes in in vivo myoelectric activity. Ricin treatment increased mean slow-wave amplitude but not frequency or resting membrane potential. Prolonged electrical field stimulation evoked a hyperpolarization during the stimulus train and a depolarization on cessation of stimulation. In the presence of atropine, the depolarization was larger in ricin-treated tissue than in control tissue, showing that ileitis enhanced noncholinergic excitation. The nitric oxide synthase inhibitor N-nitro-L-arginine methyl ester reduced the hyperpolarization in ricin-treated but not in control tissue, suggesting that inflammation increased nitric oxide-mediated inhibition. Substance P desensitization reduced noncholinergic excitation and mean slow-wave amplitude only in ricin-treated tissue, demonstrating that changes in these parameters during inflammation resulted from increased release of, or sensitivity to, tachykinins. These data suggest that acute ileitis alters tachykinin- and nitric oxide-mediated neurotransmission that may affect the normal pattern of ileal motility and/or sensory reflexes.