The gene FLORAL ORGAN NUMBER1 regulates floral meristern size in rice and encodes a leucine-rich repeat receptor kinase orthologous to Arabidopsis CLAVATA1
The gene FLORAL ORGAN NUMBER1 regulates floral meristern size in rice and encodes a leucine-rich repeat receptor kinase orthologous to Arabidopsis CLAVATA1
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DOI：
10.1242/dev.01441
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发表时间：
2004-11-01
期刊：
影响因子：
4.6
通讯作者：
Hirano, HY
中科院分区：
文献类型：
--
作者：
Suzaki, T;Sato, M;Hirano, HY
花器官数量的调节与花分生组织的大小密切相关。水稻花器官Number1(FON1)基因的突变导致水稻花分生组织扩大，导致所有花器官的数量增加。异位花器官在每个器官的轮生和/或在形成的附加轮生中发育。内花器官比外花器官受到的影响更严重。许多心皮原基发育不定，在几乎成熟的花中，未分化的分生组织留在中心。与这一结果一致的是，水稻分生组织不确定细胞的分子标记OSH1继续在该区域表达。尽管花分生组织受到Fon1-2突变的强烈影响，营养分生组织和花序分生组织在很大程度上是正常的，即使在这个强大的等位基因中也是如此。我们通过定位克隆的方法分离了FON1基因，发现它编码一个与拟南芥中CLAVATA1(CLV1)最相似的富含亮氨酸重复序列的受体样蛋白。这表明，一个类似于CLV信号系统的调节拟南芥分生组织维持的途径在禾本科植物中是保守的。与主要在茎分生组织的L3层表达的CLV1不同，FON1在整个花分生组织中都有表达，这表明可能需要对CLV信号通路进行微小的修改才能维持水稻的花分生组织。此外，在负责水稻地上部分发育的所有分生组织中都检测到FON1转录本，这表明与FON1共享功能冗余的基因在营养分生组织和花序分生组织中起作用，以掩盖Fon1突变的影响。
The regulation of floral organ number is closely associated with floral meristem size. Mutations in the gene FLORAL ORGAN NUMBER1 (FON1) cause enlargement of the floral meristem in Oryza sativa (rice), resulting in an increase in the number of all floral organs. Ectopic floral organs develop in the whorl of each organ and/or in the additional whorls that form. Inner floral organs are more severely affected than outer floral organs. Many carpel primordia develop indeterminately, and undifferentiated meristematic tissues remain in the center in almost-mature flowers. Consistent with this result, OSH1, a molecular marker of meristematic indeterminate cells in rice, continues to be expressed in this region. Although floral meristems are strongly affected by the fon1-2 mutation, vegetative and inflorescence meristems are largely normal, even in this strong allele. We isolated the FON1 gene by positional cloning and found that it encodes a leucine-rich repeat receptor-like kinase most similar to CLAVATA1 (CLV1) in Arabidopsis thaliana. This suggests that a pathway similar to the CLV signaling system that regulates meristem maintenance in Arabidopsis is conserved in the grass family. Unlike CLV1, which is predominantly expressed in the L3 layer of the shoot meristem, FON1 is expressed throughout the whole floral meristem, suggesting that small modifications to the CLV signaling pathway may be required to maintain the floral meristem in rice. In addition, FON1 transcripts are detected in all meristems responsible for development of the aerial part of rice, suggesting that genes sharing functional redundancy with FON1 act in the vegetative and inflorescence meristems to mask the effects of the fon1 mutation.