Phylogeny of the phototrophic rhizobium strain BTAi1 by polymerase chain reaction-based sequencing of a 16S rRNA gene segment
Phylogeny of the phototrophic rhizobium strain BTAi1 by polymerase chain reaction-based sequencing of a 16S rRNA gene segment
复制标题
基于聚合酶链反应的 16S rRNA 基因片段测序研究光养根瘤菌菌株 BTAi1 的系统发育
作者：
J. Young;H. L. Downer;B. Eardly
编码16 S rRNA的DNA的260-bp片段，对应于大肠杆菌序列中的位置44至337，通过聚合酶链反应扩增并从变形菌纲的α亚部中的13种细菌（根瘤菌和紫色光养生物）中的每一种进行测序。从测序片段的差异计算的系统发育树符合我们的预期，根据其他先前发表的数据。BTAi 1（最近描述的豆科植物Aeschynomene的光养共生体）的序列和自由生活的光养植物Rhodoptera palustris的序列都属于大豆共生体Bradyrhizobium japonicum菌株之间发现的变异范围。这表明，将所有这些生物体纳入一个属是适当的。
A 260-bp segment of the DNA that encodes 16S rRNA, corresponding to positions 44 to 337 in the Escherichia coli sequence, was amplified by the polymerase chain reaction and sequenced from each of 13 bacteria (rhizobia and purple phototrophs) in the alpha subdivision of the class Proteobacteria. The phylogenetic tree calculated from differences in the sequenced segment conforms well to our expectations based on other previously published data. The sequence from BTAi1 (a recently described phototrophic symbiont of the legume Aeschynomene) and that from the free-living phototroph Rhodopseudomonas palustris both fall within the range of variation found among strains of the soybean symbiont Bradyrhizobium japonicum. This suggests that it would be appropriate to include all of these organisms in a single genus.
DOI：
10.1073/pnas.82.20.6955
发表时间：
1985-01-01
影响因子：
11.1
作者：
LANE, DJ;PACE, B;PACE, NR
通讯作者：
PACE, NR