Salvianolic acid B promotes neural differentiation of induced pluripotent stem cells via PI3K/AKT/GSK3β/β-catenin pathway
Salvianolic acid B promotes neural differentiation of induced pluripotent stem cells via PI3K/AKT/GSK3β/β-catenin pathway
复制标题
丹酚酸 B 通过 PI3K/AKT/GSK3beta/beta-catenin 途径促进诱导多能干细胞的神经分化。
DOI：
10.1016/j.neulet.2018.02.007
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发表时间：
2018-04-03
影响因子：
2.5
通讯作者：
Rong, Limin
中科院分区：
文献类型：
--
作者：
Shu, Tao;Liu, Chang;Rong, Limin
丹酚酸B（Sal B）是主要从中药丹参中提取的水溶性成分，具有潜在的抗炎、抗氧化和抗凋亡作用，可保护神经细胞。在这里，我们探讨了 Sal B 对促进诱导多能干细胞 (iPSC) 分化为神经干细胞 (NSC)，进而进一步分化为神经元的作用和机制。在 iPSC 的神经分化过程中，将 Sal B 或磷脂酰肌醇 3 激酶 (PI3K) 抑制剂 (LY294002) 添加到培养基中。 Sal B 显着改善了 iPSC 衍生的 NSC 和神经元的增殖。此外，Sal B 显着刺激 PI3K/AKT/GSK3 β/β-catenin 通路。然而，LY294002 减弱了 Sal B 诱导的增加。因此，这些结果表明 Sal B 通过 PI3K/AKT/GSK3 β/β-连环蛋白途径显着增强 iPSC 的神经分化。
Salvianolic acid B (Sal B), a water-soluble component mainly extracted from the traditional Chinese medicine Salvia miltiorrhiza, has potential anti-inflammatory, anti-oxidative and anti-apoptotic actions to protect neural cells. Here, we explore the effects and mechanisms of Sal B on the promotion of differentiation of induced pluripotent stem cells (iPSCs) into neural stem cells (NSCs), then further into neurons. During the processes of neural differentiation of iPSCs, Sal B or a phosphatidylinositide 3 kinase (PI3K) inhibitor (LY294002) were added to the medium. Sal B substantially improved proliferation of iPSC-derived NSCs and neurons. Furthermore, Sal B significantly stimulated PI3K/AKT/GSK3 beta/beta-catenin pathway. However, LY294002 attenuated the Sal B-induced increase. Therefore, these outcomes suggest that Sal B markedly enhances neural differentiation of iPSCs via the PI3K/AKT/GSK3 beta/beta-catenin pathway.