Development of a point-of-care test for the detection of MDMA in latent fingerprints using surface plasmon resonance and lateral flow technology
Development of a point-of-care test for the detection of MDMA in latent fingerprints using surface plasmon resonance and lateral flow technology
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使用表面等离振子共振和侧流技术开发用于检测潜指纹中 MDMA 的即时测试
DOI：
10.1002/dta.3196
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发表时间：
2021
影响因子：
2.9
通讯作者：
Pollard C
中科院分区：
文献类型：
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作者：
Pollard C
迄今为止，尚未探索潜在指纹（LFP）中3，4-亚甲二氧基甲基苯丙胺（MDMA，摇头丸，“E”）的特定床旁检测（POCT）。其他POCT通过检测药物作为交叉反应物而不是目标分析物来识别汗液中的MDMA，从而降低了测试的灵敏度。本研究的目的是利用表面等离子体共振（SPR）和侧流免疫分析（LFA）技术设计一种灵敏的POCT检测LFP中的MDMA。使用前一种技术鉴定了高亲和力抗体结合对，认为该对适合于LFA。检测荧光标记抗体和抗原浓度的滴定，以识别添加MDMA后信号的急剧下降，从而明确区分阴性和阳性结果。我们通过用MDMA和一组与MDMA具有相似化学结构的药物产生剂量反应曲线来测试LFA。这些是通过用增加水平的药物（0-400 pg/10 μl MDMA; 0- 10，000 pg/10 μl交叉反应物）加标LFA产生的。使用试剂盒读数器测量荧光测试信号。与40 pg/10 μl相比，临界值（阈值）60 pg/10 μl计算出更好的检测盒性能（灵敏度1.00，特异性0.95和准确度0.98）。最大的交叉反应物是PMMA（250%），其次是MDEA（183%），MBDB（167%），MDA（16%）和甲基苯丙胺（16%）。成功地设计了一种灵敏的LFP筛选工具，无需样品制备。
To date, a specific point‐of‐care test (POCT) for 3,4‐methylenedioxymethamphetamine (MDMA, ecstasy, ‘E’) in latent fingerprints (LFPs) has not been explored. Other POCTs identify MDMA in sweat by detecting the drug as a cross‐reactant rather than target analyte, thus decreasing the test's sensitivity. The study's aim was to design a sensitive POCT for the detection of MDMA in LFPs using surface plasmon resonance (SPR) and lateral flow immunoassay (LFA) technology. A high‐affinity antibody binding pair was identified using the former technique, deeming the pair suitable for a LFA. Titrations of fluorescently labelled antibody and antigen concentrations were tested to identify a sharp drop‐in signal upon the addition of MDMA to allow a clear distinction between negative and positive outcomes. We trialled the LFA by producing dose response curves with MDMA and a group of drugs that share a similar chemical structure to MDMA. These were generated through spiking the LFA with increasing levels of drug (0–400 pg/10 μl of MDMA; 0–10,000 pg/10 μl of cross‐reactant). Fluorescent test signals were measured using a cartridge reader. The cut‐off (threshold) 60 pg/10 μl calculated better cartridge performance (1.00 sensitivity, 0.95 specificity and 0.98 accuracy), when compared with 40 pg/10 μl. The biggest cross‐reactant was PMMA (250%), followed by MDEA (183%), MBDB (167%), MDA (16%) and methamphetamine (16%). A sensitive LFP screening tool requiring no sample preparation was successfully designed.