Characterization of Streptococcus constellatus strains recovered from a brain abscess and periodontal pockets in an immunocompromised patient
Characterization of Streptococcus constellatus strains recovered from a brain abscess and periodontal pockets in an immunocompromised patient
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DOI：
10.1902/jop.2004.75.12.1720
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发表时间：
2004-12-01
影响因子：
4.3
通讯作者：
Olsen, I
中科院分区：
文献类型：
--
作者：
da Silva, RM;Caugant, DA;Olsen, I
背景：有许多关于脑脓肿的报告提示其病因为牙源性。然而，还没有努力比较脑脓肿分离株与口腔分离株使用高度区分的方法。我们报告一个由星座链球菌引起的脑脓疡，在一个免疫功能低下的病人，方法：采用一种表型和三种遗传学方法对脑脓肿和口腔分离株进行比较（限制性片段长度多态性[RFLP]核糖体分型和随机扩增多态性DNA [RAPD]）指纹技术。表型方法和RFLP显示脑和牙周分离物之间的相同的配置文件，而核糖分型和RAPD显示非常接近的相似性，只有一个带的差异，在三个核糖型和三个多态性的RAPD之一。牙周袋中通过基因重组事件进行的基因转移可能是导致S.有可能导致远处部位（脑）脓肿的星座状血管。牙源性来源的重要性作为潜在的病灶感染脑脓肿进行了讨论。
Background: There have been a number of reports of brain abscesses suggesting an odontogenic etiology. However, no efforts have been made to compare brain abscess isolates with isolates from the oral cavity using highly discriminative methods. We report a brain abscess caused by Streptococcus constellatus in an immuno-compromised patient where oral infection (periodontitis) was suspected to be implicated.Methods: The brain abscess and oral isolates were compared by means of one phenotypic and three genetic (restriction fragment length polymorphism [RFLP] ribotyping, and random amplifled polymorphic DNA [RAPD]) fingerprinting techniques.Results: The phenotypic method and RFLP showed identical profiles between brain and periodontal isolates, while ribotyping and RAPD showed very close similarity, with only one band difference in one of the three ribotypes and in one of the three polymorphic RAPD.Conclusions: Gene transfer by genetic recombinational events in the periodontal pocket might have been responsible for the emergence of a strain variant of S. constellatus that had the potential to cause an abscess at a distant site (brain). The importance of odontogenic sources as potential foci of infection for brain abscesses is discussed.