Nucleotide sequence and expression of relaxin-like gonad-stimulating peptide gene in starfish Asterina pectinifera
Nucleotide sequence and expression of relaxin-like gonad-stimulating peptide gene in starfish Asterina pectinifera
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DOI：
10.1016/j.ygcen.2015.06.017
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发表时间：
2016-02-01
影响因子：
2.7
通讯作者：
Mita, Masatoshi
中科院分区：
文献类型：
--
作者：
Haraguchi, Shogo;Ikeda, Narumi;Mita, Masatoshi
海星性腺刺激物质（GSS）是唯一已知的负责最终配子成熟的无脊椎动物肽激素，使其功能类似于脊椎动物中的促性腺激素。由于GSS属于松弛素样肽家族，我们建议将海星促性腺激素重新命名为松弛素样性腺刺激肽（RGP）。本研究对海燕（Asterina pectinifera）RGP基因的一级结构和表达调控进行了研究。RGP全长3896 bp，分为两个外显子，外显子1为208 bp，外显子2为2277 bp，内含子为1411 bp。在RGP编码DNA序列的5 ′上游存在启动子序列CAAT和TATA盒。转录本长度为2485个碱基（B）。AAUAAA多聚腺苷酸化信号出现在距离终止密码子2kb以上的3 '非翻译区。这表明只有14%的RGP mRNA被翻译成肽，因为开放阅读框的大小为351 B。用RGP特异性引物进行实时荧光定量PCR分析，结果表明RGP mRNA在桡神经中有高水平表达。在贲门胃中也观察到表达，但水平较低，在性腺、幽门盲囊和管足中检测到痕量水平。这一结果表明，RGP基因主要在A.果胶(C)2015 Elsevier Inc. All rights reserved.
Starfish gonad-stimulating substance (GSS) is the only known invertebrate peptide hormone responsible for final gamete maturation, rendering it functionally analogous to gonadotropins in vertebrates. Because GSS belongs to the relaxin-like peptide family, we propose renaming for starfish gonadotropic hormone as relaxin-like gonad-stimulating peptide (RGP). This study examined the primary structure and expression regulation of the RGP gene in starfish Asterina pectinifera. RGP consisted of 3896 base pairs (bp) divided over two exons, exon 1 of 208 bp and exon 2 of 2277 bp, and one intron of 1411 bp. Promoter sequences, CAAT and TATA boxes, were present in the 5'-upstream region of the coding DNA sequence of RGP. The transcript was 2485 bases (b) in length. The AAUAAA polyadenylation signal was found in 3'-untranslated region over 2 kb away from the stop codon. This showed that only 14% of the RGP mRNA was translated into the peptide, because a size of the open-reading frame was 351 b. Furthermore, an analysis by using real-time quantitative PCR with specific primers for RGP showed that mRNA of RGP was expressed at high levels in the radial nerves. Expression was also observed in the cardiac stomachs, although the level was low, and trace levels were detected in the gonads, pyloric caeca and tube feet. This result suggests that the RGP gene is transcribed mainly in the radial nerves of A. pectinifera. (C) 2015 Elsevier Inc. All rights reserved.