Ultrahigh speed spectral-domain optical coherence microscopy
Ultrahigh speed spectral-domain optical coherence microscopy
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DOI：
10.1364/boe.4.001236
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发表时间：
2013-08-01
影响因子：
3.4
通讯作者：
Fujimoto, James G.
中科院分区：
文献类型：
--
作者：
Lee, Hsiang-Chieh;Liu, Jonathan J.;Fujimoto, James G.
我们展示了一种紧凑的超高速谱域光学相干显微镜 (SD-OCM) 系统，用于在 840 nm 下对样本进行多尺度成像。使用高速 512 像素线扫描相机，展示了每秒 210,000 次 A 扫描的成像速度。放大倍数为 10 倍、20 倍和 40 倍的可互换水浸物镜可在多种尺寸尺度上提供共同配准的正面细胞分辨率成像。体积 OCM 数据集和正面 OCM 图像在正常和病理性人类结肠和肾脏标本上进行了体外验证，轴向分辨率类似于 4.2 μm，横向分辨率类似于 2.9 μm (10x)、类似于 1.7 μm (20x) 和组织中的 1.1 μm (40x)。此外，还使用图像镶嵌演示了在扩展视场 (FOV) 上以高数值孔径获取的正面 OCM 图像。展示了不同横向和轴向分辨率的正面 OCM 图像之间的比较，这有助于设计和评估不同分辨率下傅里叶域 OCM 系统的成像性能。 (C) 2013 美国光学学会
We demonstrate a compact, ultrahigh speed spectral-domain optical coherence microscopy (SD-OCM) system for multiscale imaging of specimens at 840 nm. Using a high speed 512-pixel line scan camera, an imaging speed of 210,000 A-scans per second was demonstrated. Interchangeable water immersion objectives with magnifications of 10x, 20x, and 40x provided co-registered en face cellular-resolution imaging over several size scales. Volumetric OCM data sets and en face OCM images were demonstrated on both normal and pathological human colon and kidney specimens ex vivo with an axial resolution of similar to 4.2 mu m, and transverse resolutions of similar to 2.9 mu m (10x), similar to 1.7 mu m (20x), and similar to 1.1 mu m (40x) in tissue. In addition, en face OCM images acquired with high numerical aperture over an extended field-of-view (FOV) were demonstrated using image mosaicking. Comparison between en face OCM images among different transverse and axial resolutions was demonstrated, which promises to help the design and evaluation of imaging performance of Fourier domain OCM systems at different resolution regimes. (C) 2013 Optical Society of America