Potential Significance of Circular RNA in Human Placental Tissue for Patients with Preeclampsia
Potential Significance of Circular RNA in Human Placental Tissue for Patients with Preeclampsia
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人胎盘组织中环状 RNA 对先兆子痫患者的潜在意义
DOI：
10.1159/000447842
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发表时间：
2016-01-01
影响因子：
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通讯作者：
Jia, Ruizhe
中科院分区：
文献类型：
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作者：
Qian, Yating;Lu, Yuanqing;Jia, Ruizhe
目的：本研究旨在鉴定环状rna （circular RNAs, circRNAs）在妊娠子痫前期（pre子痫，PE）孕妇胎盘组织中的不同表达，为PE病理机制的研究提供新的途径。方法：在本研究中，我们收集了40个PE患者的胎盘组织和35个胎龄匹配的早产患者的胎盘组织。使用Arraystar circRNA微阵列技术（KANGCHEN, Shanghai, China）分析circRNA的差异表达。根据两组circRNA的基本内容及其折叠变化，并考虑到每个候选circRNA设计的发散引物的实用性，我们选择了三个上调的circRNA， hsa_circRNA_100782， hsa_circRNA_102682和hsa_circRNA_104820来验证数据。采用实时定量逆转录聚合酶链反应（qRT-PCR）估计两组Ct值。我们进一步用配对t检验和受试者工作特征（ROC）曲线来评估差异。结果：发现许多环状rna在PE胎盘组织中与对照组存在差异表达；其中，143个环状rna上调，158个下调。与对照组相比，实验组hsa_circRNA_100782 （p < 0.05）、hsa_circRNA_102682 （p < 0.05）、hsa_circRNA_104820 （p < 0.0001）的表达水平均显著上调。最后，我们进行了文献比较，以预测PE期间circRNA功能的可能机制。结论：circRNA在胎盘PE组织中的表达与对照组相比有显著差异。根据circRNA芯片的结果和现有的论文，circRNA可能作为miRNA海绵参与PE的发病机制；这种可能性需要在未来的研究中进一步调查。
Aims: This study aimed to identify the different expression of circular RNAs (circRNAs) in the placental tissues of pregnant women with preeclampsia (PE) and to provide a new avenue of research regarding the pathological mechanisms of PE. Methods: In this study, we collected 40 placental tissues from PE patients and 35 placental tissues from gestational age-matched patients who gave premature birth. Arraystar circRNA Microarray Technology (KANGCHEN, Shanghai, China) was used to analyze the differential expression of circRNAs. According to the basic content of circRNAs in the two groups and their fold changes and due to the practicability of the designed divergent primers of each candidate circRNA, we selected three up-regulated circRNAs, hsa_circRNA_100782, hsa_circRNA_102682 and hsa_circRNA_104820, to validate the data. Real-time quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR) was utilized to estimate the Ct values in both groups. We further evaluated the differences with a paired t-test and a receiver operating characteristic (ROC) curve. Results: Many circRNAs were found to be differentially expressed in PE placental tissues versus their controls; of these, 143 circRNAs were up-regulated and 158 were down-regulated. The expression levels of hsa_circRNA_100782 (p < 0.05), hsa_circRNA_102682 (p < 0.05), and hsa_circRNA_104820 (p < 0.0001) were validated as significantly up-regulated in the experimental group compared with the controls. Finally, we performed a literature comparison to forecast the possible mechanisms of circRNA function during PE. Conclusion: circRNA expression significantly differed in placental PE tissues compared with controls. According to the circRNA microarray results and the existing papers, circRNAs may contribute to the pathogenesis of PE by acting as miRNA sponges; this possibility requires additional investigation in future studies.