IN-VIVO MEASUREMENT OF NEUTROPHIL ACTIVITY IN EXPERIMENTAL LUNG INFLAMMATION
IN-VIVO MEASUREMENT OF NEUTROPHIL ACTIVITY IN EXPERIMENTAL LUNG INFLAMMATION
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DOI：
10.1164/ajrccm.149.6.7516252
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发表时间：
1994-06-01
影响因子：
24.7
通讯作者：
HASLETT, C
中科院分区：
文献类型：
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作者：
JONES, HA;CLARK, RJ;HASLETT, C
正电子发射断层扫描（PET）被用来量化（18）氟脱氧葡萄糖（（18）FDG）摄取兔实验性肺炎局限于右上叶。在肺炎链球菌诱导的肺炎中，其引起持续数天的严重炎症反应才消退，（18）FDG摄取在炎症发作后15小时明显，但到48小时几乎没有摄取。在博来霉素损伤中，其从急性炎症阶段发展到慢性炎症和瘢痕形成，（18）PET可检测到的FDG摄取持续长达21天。链球菌滴注后15小时和博莱霉素滴注后2周静脉注射[H-3]脱氧葡萄糖后组织切片的放射自显影显示，在两种模型中，脱氧葡萄糖摄取均定位于中性粒细胞。在链球菌模型中，当发生主要的中性粒细胞迁移时，在6小时几乎没有（18）FGD信号。在15小时，[H-3]脱氧葡萄糖标记的中性粒细胞存在于气隙中，但不在肺泡隔中，这表明脱氧葡萄糖信号反映了迁移后的中性粒细胞事件，可能是呼吸爆发。因此，（18）FDG摄取的PET可能为在其他难以到达的部位进行中性粒细胞活性的体内研究提供一种新颖且易于重复的非侵入性方法。
Positron emission tomography (PET) was used to quantify (18)fluorodeoxyglucose ((18)FDG) uptake in rabbits with experimental pneumonitis localized to the right upper lobe. In Streptococcus pneumoniae-induced pneumonia, which causes a profound inflammatory response lasting several days before it resolves,(18)FDG uptake was pronounced at 15 h after the onset of inflammation, but by 48 h there was little uptake. in bleomycin injury, which progresses from an acute inflammatory stage to chronic inflammation and scarring, (18)FDG uptake detectable by PET persisted for up to 21 d. Autoradiography of histologic sections after intravenous administration of [H-3]deoxyglucose 15 h after streptococcal instillation and 2 wk after bleomycin instillation showed that, in both models, deoxyglucose uptake was localized to neutrophils. In the streptococcal model there was little (18)FGD signal at 6 h, when major neutrophil migration occurs. At 15 h, [H-3]deoxyglucose-labeled neutrophils were present in the airspaces but not in the alveolar septa, suggesting that the deoxyglucose signal reflected a postmigratory neutrophil event, probably the respiratory burst. Thus, PET of (18)FDG uptake may provide a novel and readily repeatable, noninvasive approach to the in vivo study of neutrophil activity at otherwise inaccessible sites.