GSH-1 - A NOVEL MURINE HOMEOBOX GENE EXPRESSED IN THE CENTRAL-NERVOUS-SYSTEM
GSH-1 - A NOVEL MURINE HOMEOBOX GENE EXPRESSED IN THE CENTRAL-NERVOUS-SYSTEM
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DOI：
10.1002/aja.1002030306
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发表时间：
1995-07-01
影响因子：
2.5
通讯作者：
POTTER, SS
中科院分区：
文献类型：
--
作者：
VALERIUS, MT;LI, H;POTTER, SS
我们报告的GSH-1，一个新的小鼠同源异型盒基因的特性。北方印迹分析显示，在胚胎发育的第10.5、11.5和12.5天存在一个大小约为2kb的转录本。该cDNA序列编码富含脯氨酸的基序、多聚丙氨酸区和同源结构域，与簇状Hox基因编码的同源结构域具有很强的同源性。采用全包埋法和连续切片原位杂交法测定E8.5 ~ E13.5天GSH-1的表达模式。GSH-1转录仅限于中枢神经系统。表达存在于神经管和后脑中，作为神经上皮组织内的两个连续的、双侧对称的条纹。在中脑中，表达被视为跨越最前部的条带。在丘脑和下丘脑的原基中以及在视柄、视隐窝和神经节隆起中也有间脑表达。此外，通过使用含有Gsh-1同源结构域的融合蛋白，我们已经确定Gsh-1同源蛋白的共有DNA结合位点为GC（T）/（A）（C）/（C）ATTA（G）/（A）。(C)1995 Wiley-Liss，Inc.
We report the characterization of Gsh-1, a novel murine homeobox gene. Northern blot analysis revealed a transcript of approximately 2 kb in size present at embryonic days 10.5, 11.5, and 12.5 of development, The cDNA sequence encoded a proline rich motif, a polyalanine tract, and a homeodomain with strong homology to those encoded by the clustered Hox genes. The Gsh-1 expression pattern was determined for days E8.5 to E13.5 by whole mount and serial section in situ hybridizations. Gsh-1 transcription was restricted to the central nervous system. Expression is present in the neural tube and hindbrain as two continuous, bilaterally symmetrical stripes within neural epithelial tissue. In the mesencephalon, expression is seen as a band across the most anterior portion. There is also diencephalon expression in the anlagen of the thalamus and the hypothalamus as well as in the optic stalk, optic recess, and the ganglionic eminence. Moreover, through the use of fusion proteins containing the Gsh-1 homeodomain, we have determined the consensus DNA binding site of the Gsh-1 homeoprotein to be GC(T)/(A)(C)/(C)ATTA(G)/(A). (C) 1995 Wiley-Liss, Inc.