Complete multipoint sib-pair analysis of qualitative and quantitative traits.
Complete multipoint sib-pair analysis of qualitative and quantitative traits.
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DOI：
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发表时间：
1995-08
影响因子：
9.8
通讯作者：
L. Kruglyak;E. Lander
中科院分区：
文献类型：
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作者：
L. Kruglyak;E. Lander
同胞对分析是一个越来越重要的工具，遗传解剖的复杂性状。目前的同胞对分析方法主要是基于一次研究一个个体遗传标记，因此无法使用多点连锁分析提供的完整遗传信息。在本文中，我们描述了如何提取完整的多点遗传信息的每个同胞对。然后，我们描述的方法，使用这些信息来映射影响性状的基因座，从而提供了一个统一的方法来定性和定量性状。具体来说，完整的多点方法（1）排除映射的质量性状;（2）最大似然映射的质量性状;（3）信息内容映射，显示在何种程度上所有的遗传信息已被提取在基因组中的每个位置;（4）数量性状映射，通过两个参数的方法和一个非参数的方法。此外，我们还探讨了标记密度、标记多态性和亲本可用性对研究信息含量的影响。我们已经实现了一个新的计算机程序包，MAPMAKER/SIBS的分析方法。使用该计算机软件包，可以在几分钟内对数百个同胞对中的数十个标记进行完整的多点分析。
Sib-pair analysis is an increasingly important tool for genetic dissection of complex traits. Current methods for sib-pair analysis are primarily based on studying individual genetic markers one at a time and thus fail to use the full inheritance information provided by multipoint linkage analysis. In this paper, we describe how to extract the complete multipoint inheritance information for each sib pair. We then describe methods that use this information to map loci affecting traits, thereby providing a unified approach to both qualitative and quantitative traits. Specifically, complete multipoint approaches are presented for (1) exclusion mapping of qualitative traits; (2) maximum-likelihood mapping of qualitative traits; (3) information-content mapping, showing the extent to which all inheritance information has been extracted at each location in the genome; and (4) quantitative-trait mapping, by two parametric methods and one nonparametric method. In addition, we explore the effects of marker density, marker polymorphism, and availability of parents on the information content of a study. We have implemented the analysis methods in a new computer package, MAPMAKER/SIBS. With this computer package, complete multipoint analysis with dozens of markers in hundreds of sib pairs can be carried out in minutes.