Isolation of genomic DNA using magnetic cobalt ferrite and silica particles
Isolation of genomic DNA using magnetic cobalt ferrite and silica particles
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DOI：
10.1016/j.chroma.2004.08.090
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发表时间：
2004-11-12
影响因子：
4.1
通讯作者：
Benes, MJ
中科院分区：
文献类型：
--
作者：
Prodelalová, J;Rittich, B;Benes, MJ
吸附分离技术作为费力的传统方法（例如基于苯酚提取程序）的替代方法已应用于 DNA 纯化。在这项工作中，我们使用了两种类型的颗粒：二氧化硅和钴铁氧体（未改性或用含有弱碱性氨基乙基、氨基苯基或藻酸的试剂改性）。使用来自鸡红细胞的 DNA 和从细菌乳酸乳球菌中分离的 DNA 来测试颗粒的吸附/解吸特性。用不同试剂修饰的钴铁氧体颗粒用于从不同乳制品中分离 PCR 就绪细菌 DNA。 (C) 2004 Elsevier B.V. 保留所有权利。
Adsorption separation techniques as an alternative to laborious traditional methods (e.g., based on phenol extraction procedure) have been applied for DNA purification. In this work we used two types of particles: silica and cobalt ferrite (unmodified or modified with a reagent containing weakly basic aminoethyl groups, aminophenyl groups, or alginic acid). DNA from chicken erythrocytes and DNA isolated from bacteria Lactococcus lactis were used for testing of adsorption/desorption properties of particles. The cobalt ferrite particles modified with different reagents were used for isolation of PCR-ready bacterial DNA from different dairy products. (C) 2004 Elsevier B.V. All rights reserved.