Is photosynthetic transcriptional regulation in Triticum aestivum L. cv. 'TugelaDN' a contributing factor for tolerance to Diuraphis noxia (Homoptera: Aphididae)?
Is photosynthetic transcriptional regulation in Triticum aestivum L. cv. 'TugelaDN' a contributing factor for tolerance to Diuraphis noxia (Homoptera: Aphididae)?
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DOI：
10.1007/s00299-005-0001-9
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发表时间：
2006-02-01
影响因子：
6.2
通讯作者：
Cullis, CA
中科院分区：
文献类型：
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作者：
Botha, AM;Lacock, L;Cullis, CA
麦长管蚜(Diuraphim noxiia，RWA)是南非和大多数其他小麦种植国的主要害虫。作为一种刺吸性昆虫，RWA将它们的口针插入韧皮部筛分元件，并以韧皮部汁液为食。这种取食会导致抗病品种的坏死病，或感病品种的叶片脱色和死亡。为了扩大我们对植物对RWA取食反应的理解，我们从抑制消减杂交(SSH)文库中合成并分析了表达序列标签(EST)。利用感病‘Tugela’和抗病‘TugelaDN’(DN1)的近等基因小麦品系以及加入系PI137739(DN1)和PI294994(DN5)构建了这些文库。对文库中200个EST的分析表明，编码基因的转录本涉及细胞维持、生长和调节、植物防御和信号、光合作用和能量产生，以及未知功能。选择这些EST，结合从其他来源获得的克隆，在定制的阵列上用于研究256个候选小麦序列的表达谱，这些候选序列可能与植物对RWA的防御有关。所选择的序列包括带有核苷酸结合位点(NBS)基序的小麦基因组克隆、快速扩增cDNA末端聚合酶链式反应(RACE-PCR)和来自RWA诱导文库的cDNA克隆。通过代表性差异分析(RDA)获得的怀疑参与非生物胁迫反应的基因组香蕉和亚麻克隆也被发现在微阵列载玻片上。在侵染后0、2、5和8d，将斑点定制阵列与从抗病品种‘Tugela DN’分离的cDNA杂交，然后用Cy3或Cy5荧光染料进行标记。随后使用DNA微阵列、RT-PCR和Northern Blot分析的表达谱鉴定了29个与摄食反应相关的转录本。这些转录本编码的蛋白质具有直接防御和信号传递、氧化爆发、细胞壁降解、细胞维持、光合作用和能量产生等功能。结果表明，当植物受到RWA攻击时，植物协调调节基因的表达。据推测，NBS-LRR蛋白在受体识别和信号转导中起重要作用，使植物能够克服RWA取食造成的逆境。进一步认为，在处理RWA取食时，维持光合作用功能并产生能量的能力是确保抗病品种存活的决定因素之一。
Diuraphis noxia (Russian wheat aphid, RWA) is a major pest on wheat in South Africa and most other wheat growing countries. Being a probing-sucking insect, RWAs insert their stylets into the phloem sieve elements and feed on the phloem sap. This feeding causes necrotic lesions in resistant varieties, or decoloration of leaves and death in susceptible varieties. In an effort to broaden our understanding on the response of the plant to RWA feeding, we synthesized and analyzed expressed sequence tags (ESTs) from suppression subtractive hybridization (SSH) libraries. These libraries were constructed using near isogenic wheat lines susceptible 'Tugela' and resistant 'TugelaDN'(Dn1) to RWA, as well as accession lines PI137739 (Dn1) and PI294994 (Dn5). Analysis of 200 ESTs from the libraries revealed the involvement of transcripts encoding genes involved in cell maintenance, growth and regulation, plant defense and signaling, photosynthesis and energy production, and of unknown function. A selection of these ESTs, in combination with clones obtained from other sources, were used on a custom array to study the expression profiles of 256 candidate wheat sequences putatively involved in plant defense against RWA. The selected sequences included wheat genomic clones with putative nucleotide binding site (NBS) motifs, rapid amplification of cDNA ends PCR (RACE-PCR), and cDNA clones from RWA induced libraries. Genomic banana and flax clones that were obtained using representative difference analysis (RDA), and suspected to be involved in abiotic stress responses, were also spotted onto the microarray slides. The spotted custom arrays were then hybridized against cDNA isolated from a resistant cultivar 'Tugela DN' on 0, 2, 5, and 8 days after infestation, post-labeled with Cy3- or Cy5-fluorescent dyes. The subsequent expression profiling using DNA microarray, RT-PCR, and Northern Blot analysis identified 29 transcripts associated with the feeding response. These transcripts encoded proteins functioning in direct defense and signaling, oxidative burst, cell wall degradation, cell maintenance, photosynthesis, and energy production. Results indicate that plants co-ordinately regulate gene expression when attacked by RWA. It is hypothesized that the NBS-LRR proteins are important in receptor recognition and signaling, which enable the plant to overcome the stresses inflicted by RWA feeding. It is further suggested that the ability to maintain photosynthetic function with resultant energy production is one of the determining factors ensuring the survival of the resistant varieties when coping with the RWA feeding.