Directing cell migration and organization via nanocrater-patterned cell-repellent interfaces.
Directing cell migration and organization via nanocrater-patterned cell-repellent interfaces.
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DOI：
10.1038/nmat4342
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发表时间：
2015-09
期刊：
影响因子：
41.2
通讯作者：
Healy KE
中科院分区：
文献类型：
--
作者：
Jeon H;Koo S;Reese WM;Loskill P;Grigoropoulos CP;Healy KE
虽然细胞和纳米结构界面之间的粘附相互作用已经被广泛研究，但关于纳米结构界面如何通过指导细胞迁移和细胞集落组织来排斥细胞的数据缺乏。在这里，通过使用多光子消融光刻技术对不同纵横比和间距的纳米级陨石坑表面进行图案设计，我们发现这些表面改变了细胞的聚焦粘附大小和分布，从而影响了细胞的形态、迁移和最终定位。我们还发现，纳米颗粒的间距可以破坏成熟的局灶粘连的形成，有利于细胞向更高的音调区域迁移，这为形成稳定的局灶粘连提供了更多的平面面积。此外，通过设计具有可变间距但恒定纳米尺寸的表面，我们能够创建圆形和条纹细胞图案。我们的表面图案化方法不涉及化学处理，可以应用于各种材料，代表了一种控制表面细胞行为的简单方法。
Although adhesive interactions between cells and nanostructured interfaces have been studied extensively, there is a paucity of data on how nanostructured interfaces repel cells by directing cell migration and cell-colony organization. Here, by using multiphoton ablation lithography to pattern surfaces with nanoscale craters of various aspect ratios and pitches, we show that the surfaces altered the cells’ focal-adhesion size and distribution, thus affecting cell morphology, migration and ultimately localization. We also show that nanocrater pitch can disrupt the formation of mature focal adhesions to favour the migration of cells toward higher-pitched regions, which present increased planar area for the formation of stable focal adhesions. Moreover, by designing surfaces with variable pitch but constant nanocrater dimensions, we were able to create circular and striped cellular patterns. Our surface-patterning approach, which does not involve chemical treatments and can be applied to various materials, represents a simple method to control cell behaviour on surfaces.