Effects of selenium on altered mechanical and electrical cardiac activities of diabetic rat
Effects of selenium on altered mechanical and electrical cardiac activities of diabetic rat
复制标题
DOI：
10.1016/j.abb.2004.03.030
复制
发表时间：
2004-06-01
影响因子：
3.9
通讯作者：
Turan, B
中科院分区：
文献类型：
--
作者：
Ayaz, M;Ozdemir, S;Turan, B
由于硒化合物可以恢复糖尿病大鼠心脏的某些代谢参数和结构变化，我们试图研究这些有益的作用是否延伸到糖尿病大鼠的心功能障碍。用链脲佐菌素(50 mg/kg体重)复制糖尿病大鼠模型，然后给予亚硒酸钠(5mmool/kg体重/d)治疗4周。记录电刺激的乳头肌等长收缩和细胞内动作电位，以及心肌细胞瞬时(I-lo)和稳态(I-ss)外向钾电流。亚硒酸钠能逆转糖尿病大鼠动作电位时程和抽动时程的延长，并使两种K+电流的幅度显著增加。用亚硒酸钠治疗的大鼠也显著增加了心脏中受抑制的酸溶巯基水平。我们的数据表明，亚硒酸钠对糖尿病大鼠心脏机械和电活动的有益影响似乎是由于减弱的K+电流的恢复，部分与细胞谷胱甘肽氧化还原循环的恢复有关。(C)2004 Elsevier Inc.保留所有权利。
Since selenium compounds can restore some metabolic parameters and structural alterations of diabetic rat heart, we were tempted to investigate whether these beneficial effects extend to the diabetic rat cardiac dysfunctions. Diabetes was induced by streptozotocin (50 mg/kg body weight) and rats were then treated with sodium selenite (5 mumol/kg body weight/day) for four weeks. Electrically stimulated isometric contraction and intracellular action potential in isolated papillary muscle strips and transient (I-lo) and steady state (I-ss) outward K+ currents in isolated cardiomyocytes were recorded. Sodium selenite treatment could reverse the prolongation in both action potential duration and twitch duration of the diabetic rats, and also cause significant increases in the diminished amplitudes of the two K+ currents. Treatment of rats with sodium selenite also markedly increased the depressed acid-soluble sulfhydryl levels of the hearts. Our data suggest that the beneficial effects of sodium selenite treatment on the mechanical and electrical activities of the diabetic rat heart appear to be due to the restoration of the diminished K+ currents, partially, related to the restoration of the cell glutathione redox cycle. (C) 2004 Elsevier Inc. All rights reserved.