A DIRECTORY OF HUMAN GERM-LINE V-CHI SEGMENTS REVEALS A STRONG BIAS IN THEIR USAGE
A DIRECTORY OF HUMAN GERM-LINE V-CHI SEGMENTS REVEALS A STRONG BIAS IN THEIR USAGE
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DOI：
10.1002/eji.1830240409
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发表时间：
1994-04-01
影响因子：
5.4
通讯作者：
WINTER, G
中科院分区：
文献类型：
--
作者：
COX, JPL;TOMLINSON, IM;WINTER, G
从单个个体的基因组DNA中，我们扩增、克隆和测序了37个人种系V-kappa片段。其中四个部分是新的。然后，我们编制了一个全面的目录，所有生殖系V-κ片段，并确定了50个不同的序列与开放阅读框架。与236个重排序列的比较显示，这些种系序列中不超过24个可以被分配为重排对应物，其中一些很少使用，只有大约11个序列经常使用。这表明表达的V-κ库主要来自有限数量的片段。最令人惊讶的是，该基因座的J（kappa）远端区域似乎很少使用：我们可以明确地将162个重排序列分配给J（kappa）近端区域的V-kappa片段，但只有5个分配给J（kappa）远端区域的片段。
From the genomic DNA of a single individual, we have amplified, cloned and sequenced 37 human germ-line V-kappa segments. Four of these segments were new. We then compiled a comprehensive directory of all germ-line V-kappa segments and identified 50 different sequences with open reading frames. Comparison with 236 rearranged sequences revealed that no more than 24 of these germ-line sequences could be assigned rearranged counterparts, that some of these were rarely used, and that only about 11 sequences are used frequently. This suggests that the expressed V-kappa repertoire is mainly derived from a limited number of segments. Most surprisingly, the J(kappa)-distal region of the locus appears to be rarely used: we could unambiguously assign 162 rearranged sequences to V-kappa segments of the J(kappa)-proximal region, but only 5 to segments of the J(kappa) -distal region.