Effects of regenerating vegetation on soil enzyme activity and microbial structure in reclaimed soils on a surface coal mine site
Effects of regenerating vegetation on soil enzyme activity and microbial structure in reclaimed soils on a surface coal mine site
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DOI：
10.1016/j.apsoil.2014.11.010
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发表时间：
2015-03-01
影响因子：
4.8
通讯作者：
He, Jizheng
中科院分区：
文献类型：
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作者：
Li, Junjian;Zhou, Xiaomei;He, Jizheng
以西北黄土高原某露天煤矿复垦地为研究对象，研究复垦情景对土壤酶活性和微生物群落的影响。土壤样品采集自裸地（CK），种植园（PL）和4个混交林（MF 1 -4）。测定了土壤理化性质、4种酶活性、微生物丰度和T-RFLP（末端限制性片段长度多态性）谱。复垦情景对土壤养分含量、酶活性和微生物群落的影响较为显著。土壤有机碳可以很好地预测酶活性、微生物丰度和组成的主要差异。土壤酶活性与真菌丰度的相关性比与细菌和古细菌的相关性更显著。混交林土壤养分含量、酶活性、微生物丰度和多样性均较高，而对照林土壤养分含量、酶活性、微生物丰度和多样性最低，说明混交林是黄土高原半干旱区适宜的生态环境。土壤细菌、古菌和真菌随复垦过程而演化，但不同复垦情景对土壤微生物丰度、多样性和组成的影响不同。这些结果表明，土壤细菌、古菌和真菌在恢复过程中发挥着不同的生态作用。(C)2014爱思唯尔有限公司版权所有。
The aim of this study was to evaluate the influence of reclaimed scenarios on soil enzyme activities and microbial community in a reclaimed surface coal mine on the Northwest Loess Plateau of China. Soil samples were collected from a bare land (CK), and a plantation (PL) and four mixed forests (MF1-4). Soil physicochemical characteristics, four enzyme activities and microbial abundance and T-RFLP (terminal restriction fragment length polymorphism) profiles were measured. Effects of reclaimed scenarios on soil nutrients content, enzyme activities and microbial community were pronounced. Soil organic carbon could be well used to predict the major differences in enzyme activities, and microbial abundance and composition. Soil enzyme activities were more significantly correlated with fungal abundance than bacterial and archaeal ones. The higher soil nutrient content, enzyme activities, and microbial abundance and diversity were from mixed forests and the lowest ones were from CK, which suggested mixed forests would be feasible scenarios in semi-arid Loess Plateau. Soil bacteria, archaea and fungi evolved with reclaimed process, but the influences of reclaimed scenarios on each domain of microbial abundance, diversity and composition were different. These findings suggested that soil bacteria, archaea and fungi play different ecological roles during restoration process. (C) 2014 Elsevier B.V. All rights reserved.