EVALUATION OF A RAPID RADIOMETRIC METHOD FOR DRUG SUSCEPTIBILITY TESTING OF MYCOBACTERIUM-TUBERCULOSIS
EVALUATION OF A RAPID RADIOMETRIC METHOD FOR DRUG SUSCEPTIBILITY TESTING OF MYCOBACTERIUM-TUBERCULOSIS
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DOI：
10.1128/jcm.13.5.908-912.1981
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发表时间：
1981-01-01
影响因子：
9.4
通讯作者：
MIDDLEBROOK, G
中科院分区：
文献类型：
--
作者：
SIDDIQI, SH;LIBONATI, JP;MIDDLEBROOK, G
用常规7H11平板法和一种新的快速放射学方法对106株结核分枝杆菌进行了药敏试验，其中7H12液体培养基含14C标记底物。比较了两种方法检测结果的快速性、敏感性和特异性。两种方法所得结果总体符合率为98%。在总共424次药检中，只有8次药检结果不一致，其中大部分是链霉素。这一新程序快速，87%的检测结果可在4天内报告，98%的检测结果可在5天内报告，而传统的间接药敏试验方法通常需要3周。这种快速辐射测量方法似乎具有常规实验室使用的潜力，值得进一步研究。
A total of 106 isolates of M. tuberculosis were tested for drug susceptibility by the conventional 7H11 plate method and by a new rapid radiometric method using special 7H12 liquid medium with 14C-labeled substrate. Results obtained by the 2 methods were compared for rapidity, sensitivity and specificity of the new test method. There was 98% overall agreement between the results obtained by the 2 methods. Of a total of 424 drug tests, only 8 drug results did not agree, mostly in the case of streptomycin. This new procedure was rapid, with 87% of the test results reportable within 4 days and 98% reportable within 5 days as compared to the usual 3 wk required with the conventional indirect susceptibility test method. The rapid radiometric method seems to have the potential for routine laboratory use and merits further investigations.