Thermo-responsive molecularly imprinted nanogels for specific recognition and controlled release of proteins
Thermo-responsive molecularly imprinted nanogels for specific recognition and controlled release of proteins
复制标题
用于蛋白质特异性识别和受控释放的热响应分子印迹纳米凝胶
DOI：
10.1039/c3sm27505a
复制
发表时间：
2013-01-01
期刊：
影响因子：
3.4
通讯作者：
Li, Bin
中科院分区：
文献类型：
--
作者：
Pan, Guoqing;Guo, Qianping;Li, Bin
智能纳米凝胶能够响应环境刺激，具有开关特性，在药物/基因传递、诊断和治疗等生物医学领域具有广阔的应用前景。在这里，我们报道了一种新型的温度响应性纳米凝胶的合成和表征，该纳米凝胶是利用分子印迹技术构建的，用于蛋白质的特定识别和控制释放。以溶菌酶为蛋白质模板，N-异丙基丙烯酰胺为主要单体，在表面活性剂十二烷基硫酸钠(十二烷基硫酸钠)的辅助下，采用水相沉淀聚合法制备了蛋白质印迹球形纳米凝胶。只需在聚合过程中调整十二烷基硫酸钠的量，纳米凝胶的尺寸就可以得到很好的控制，从几百到几十纳米不等。与非印迹纳米凝胶相比，溶菌酶印迹纳米凝胶具有更高的重结合能力、更快的重结合动力学和更高的溶菌酶特异性。重要的是，溶菌酶印迹纳米凝胶的再结合和释放特性都表现出明显的温度依赖性，在33℃附近有明显的开关转变，即温度响应性聚合物聚N-异丙基丙烯酰胺的体积相变温度。因此，我们开发了一种简单而通用的方法来制备尺寸可控、对特定生物分子具有温度响应结合/释放性能的分子印迹纳米凝胶，这可能有助于从生物分离和生物传感到药物输送和治疗的广泛应用。
Intelligent nanogels which respond to environmental stimuli with on/off characteristics hold great promise in a number of biomedical applications including drug/gene delivery, diagnostics and therapeutics. Here, we report the synthesis and characterization of a novel type of thermo-responsive nanogel built using the molecular imprinting technique for specific recognition and controlled release of proteins. Using lysozyme as the protein template and N-isopropylacrylamide as the major monomer, protein-imprinted spherical nanogel particles were readily prepared via aqueous precipitation polymerization with the aid of a surfactant, sodium dodecyl sulfate (SDS). Simply by adjusting the SDS amount during polymerization, the size of nanogels could be finely controlled, ranging from a few hundred down to a few dozen nanometers. Compared to non-imprinted counterparts, the lysozyme-imprinted nanogels possessed higher rebinding capacity, more rapid rebinding kinetics, and much higher specificity toward lysozyme. Importantly, both the rebinding and release characteristics of lysozyme-imprinted nanogels showed dramatic temperature-dependence, with clear on–off transition around 33 °C, i.e., the volume phase transition temperature of the thermo-responsive polymer poly(N-isopropylacrylamide). Therefore, we have developed a facile yet versatile approach to fabricate molecularly imprinted nanogels of well controlled sizes and thermo-responsive binding/release properties toward specific biomolecules, which may facilitate a broad spectrum of applications ranging from bioseparation and biosensing to drug delivery and therapeutics.