Monoclonal antibodies distinguish subtypes of retinal horizontal cells.
Monoclonal antibodies distinguish subtypes of retinal horizontal cells.
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单克隆抗体可区分视网膜水平细胞的亚型。
DOI：
10.1073/pnas.81.19.6255
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发表时间：
1984
影响因子：
11.1
通讯作者：
Dowling,JE
中科院分区：
文献类型：
--
作者：
Young,LH;Dowling,JE
已经鉴定出16个杂交瘤，它们分泌针对鲤鱼视网膜水平细胞的特异性抗体。根据其抗体染色模式，将杂交瘤分为三组：组I，与所有水平细胞相关的染色;组II，与最丰富的水平细胞亚型（CH 1）相关的染色;组III，与其他水平细胞亚型相关的染色。大多数的杂交瘤属于第二组;这些抗体中的一些染色整个水平细胞，但其他人只对细胞胞体特异，不染色轴突过程。我们的研究结果表明，有特定的表面分子（i）所有视网膜水平细胞，（ii）水平细胞的个别亚型，（iii）水平细胞的部分。此外，已发现识别48- 50-kDa膜蛋白的II组抗体提供了对水平细胞生长具有选择性的底物。铺在用这种抗体包被的盖玻片上的水平细胞在培养中保持健康，并延长长而精细的过程至少3周。
Sixteen hybridomas have been identified that secrete antibodies specific to horizontal cells in the carp retina. The hybridomas have been classified into three groups based on their antibody staining patterns: group I, staining associated with all horizontal cells; group II, staining associated with the most abundant subtype of horizontal cell (CH1); and group III, staining associated with other subtypes of horizontal cells. Most of the hybridomas fall in group II; some of these antibodies stain the entire horizontal cell, but others are specific only to the cell perikarya and do not stain axonal processes. Our results suggest that there are surface molecules specific (i) to all retinal horizontal cells, (ii) to individual subtypes of horizontal cells, and (iii) to portions of horizontal cells. Furthermore, a group II antibody, which recognizes a 48- to 50-kDa membrane protein, has been found to provide a substrate selective for horizontal cell growth. Horizontal cells plated on coverslips coated with this antibody remain healthy in culture and extend long and elaborate processes for at least 3 weeks.