Vaccine development in Staphylococcus aureus: taking the biofilm phenotype into consideration.
Vaccine development in Staphylococcus aureus: taking the biofilm phenotype into consideration.
复制标题
DOI：
10.1111/j.1574-695x.2010.00708.x
复制
发表时间：
2010-08
影响因子：
--
通讯作者：
Shirtliff ME
中科院分区：
文献类型：
--
作者：
Harro JM;Peters BM;O'May GA;Archer N;Kerns P;Prabhakara R;Shirtliff ME
针对病原菌的疫苗开发是一项势在必行的举措，因为细菌对当前的抗菌治疗产生了耐药性，并且很少有新的抗生素被开发出来。用于疫苗开发的候选抗原可以通过获得完整基因组而加速的多种高通量技术来鉴定。虽然在针对细菌病原体的疫苗开发方面取得了相当大的成功，但具有多种毒力因子和感染模式的许多物种在鉴定保护性抗原方面提出了合理的挑战。特别是，候选疫苗应在复杂疾病特性的背景下进行评价，无论是强直性（例如败血症和肺炎）和/或生物膜相关（例如留置医疗器械感染）。由于这些生长模式之间的表型差异，仅针对其在一种疾病状态下的功效而选择的那些候选疫苗可能无法抵抗其他感染。本文将总结细菌疫苗和佐剂的历史和类型，并概述现代抗原的发现和多微生物感染带来的并发症。最后，我们还将使用一种研究得更好的微生物物种，该物种使用差异的多因子蛋白质谱来介导一系列疾病，金黄色葡萄球菌，以概述最近在这种生物膜形成物种中疫苗开发中发现的一些问题。
Vaccine development against pathogenic bacteria is an imperative initiative as bacteria are gaining resistance to current antimicrobial therapies and few novel antibiotics are being developed. Candidate antigens for vaccine development can be identified by a multitude of high-throughput technologies that were accelerated by access to complete genomes. While considerable success has been achieved in vaccine development against bacterial pathogens, many species with multiple virulence factors and modes of infection have provided reasonable challenges in identifying protective antigens. In particular, vaccine candidates should be evaluated in the context of the complex disease properties, whether planktonic (e.g. sepsis and pneumonia) and/or biofilm associated (e.g. indwelling medical device infections). Because of the phenotypic differences between these modes of growth, those vaccine candidates chosen only for their efficacy in one disease state may fail against other infections. This review will summarize the history and types of bacterial vaccines and adjuvants as well as present an overview of modern antigen discovery and complications brought about by polymicrobial infections. Finally, we will also use one of the better studied microbial species that uses differential, multifactorial protein profiles to mediate an array of diseases, Staphylococcus aureus, to outline some of the more recently identified problematic issues in vaccine development in this biofilm-forming species.