Oral microbiome development during childhood: an ecological succession influenced by postnatal factors and associated with tooth decay
Oral microbiome development during childhood: an ecological succession influenced by postnatal factors and associated with tooth decay
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DOI：
10.1038/s41396-018-0204-z
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发表时间：
2018-09-01
期刊：
影响因子：
11
通讯作者：
Mira, Alex
中科院分区：
文献类型：
--
作者：
Dzidic, Majda;Collado, Maria C.;Mira, Alex
关于儿童早期口腔微生物群如何发育以及外部因素如何影响这一生态过程的信息很少。我们利用高通量测序技术，对90名纵向随访儿童在3、6、12、24个月和7岁时收集的唾液样本中的细菌组成进行了表征，并收集了这些儿童的临床、饮食和健康数据。细菌组成模式随着时间的推移而改变，从“早期殖民者”开始，包括链球菌和细孔菌；其他细菌属如奈瑟氏菌在1或2岁后定居。随着时间的推移，龋齿的发展与微生物组成的分化有关。cristatus链球菌似乎与发生蛀牙的风险增加有关，其作为该疾病潜在生物标志物的作用应通过物种特异性探针进行研究。与顺产的婴儿相比，剖腹产出生的婴儿最初的细菌含量是倾斜的，但随着年龄的增长，这种情况会恢复。较短的母乳喂养习惯和前2岁期间的抗生素治疗与后期明显的细菌组成相关。研究结果将口腔微生物群的发育描述为一种生态演替，在生命的第一年改变定植模式可能对儿童的口腔和全身健康产生长期影响。
Information on how the oral microbiome develops during early childhood and how external factors influence this ecological process is scarce. We used high-throughput sequencing to characterize bacterial composition in saliva samples collected at 3, 6, 12, 24 months and 7 years of age in 90 longitudinally followed children, for whom clinical, dietary and health data were collected. Bacterial composition patterns changed through time, starting with "early colonizers", including Streptococcus and Veillonella; other bacterial genera such as Neisseria settled after 1 or 2 years of age. Dental caries development was associated with diverging microbial composition through time. Streptococcus cristatus appeared to be associated with increased risk of developing tooth decay and its role as potential biomarker of the disease should be studied with species-specific probes. Infants born by C-section had initially skewed bacterial content compared with vaginally delivered infants, but this was recovered with age. Shorter breastfeeding habits and antibiotic treatment during the first 2 years of age were associated with a distinct bacterial composition at later age. The findings presented describe oral microbiota development as an ecological succession where altered colonization pattern during the first year of life may have long-term consequences for child's oral and systemic health.