Coupling two enzymes into a tandem nanoreactor utilizing a hierarchically structured MOF.
Coupling two enzymes into a tandem nanoreactor utilizing a hierarchically structured MOF.
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DOI：
10.1039/c6sc01438k
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发表时间：
2016-12-01
期刊：
影响因子：
8.4
通讯作者：
Zhou HC
中科院分区：
文献类型：
--
作者：
Lian X;Chen YP;Liu TF;Zhou HC
一种具有分级结构的金属 - 有机框架（MOF）被用于以串联方式偶联两种酶。按特定顺序逐步包封是实现这一目标的唯一方法。
一种具有分级多孔结构的金属 - 有机框架（MOF），即PCN - 888，含有三种类型的空腔，被用于将两种酶偶联到一个串联纳米反应器中。最大的空腔（6.2纳米）只能容纳一个葡萄糖氧化酶（GOx）分子。中间的空腔（5.0纳米）可以容纳且只能容纳一个辣根过氧化物酶（HRP）分子。小空腔（2.0纳米）对于GOx和HRP来说尺寸都不够，并且作为底物扩散通道保持开放。这两种酶的偶联只能通过一种具有特定顺序的独特逐步包封来实现（先包封GOx，然后是HRP）。该串联纳米反应器表现出优异的催化性能，且酶的泄漏可忽略不计。其催化活性在几个催化循环中都能很好地保持。此外，PCN - 888可以保护包封的酶免受胰蛋白酶消化，这表明该纳米反应器在体外和体内都具有稳定性。
A hierarchically structured MOF is utilized to couple two enzymes in a tandem manner. A stepwise encapsulation with a specific order is the only way to achieve this goal. A hierarchical porous metal–organic framework (MOF), PCN-888, containing three types of cavities was utilized to couple two enzymes into a tandem nanoreactor. The largest cavity (6.2 nm) can only accommodate one molecule of glucose oxidase (GOx). The intermediate cavity (5.0 nm) can accommodate one and only one molecule of horseradish peroxidase (HRP). The small cavity (2.0 nm) has sufficient size for neither GOx nor HRP, and remains open as a substrate diffusion pathway. The coupling of the two enzymes can only be achieved through a unique stepwise encapsulation with a specific order (GOx first, followed by HRP). The tandem nanoreactor shows excellent catalytic performances and negligible enzyme leaching. Its catalytic activity is well maintained within several catalytic cycles. Moreover, PCN-888 can provide protection to the encapsulated enzymes against trypsin digestion, indicating the in vitro and in vivo stability of the nanoreactor.