A novel T-DNA integration in rice involving two interchromosomal translocations
A novel T-DNA integration in rice involving two interchromosomal translocations
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DOI：
10.1007/s00299-014-1572-0
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发表时间：
2014-06-01
影响因子：
6.2
通讯作者：
Veluthambi, Karuppannan
中科院分区：
文献类型：
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作者：
Majhi, Bharat Bhusan;Shah, Jasmine M.;Veluthambi, Karuppannan
一个雄性不育的转基因水稻植株TC-19在8号染色体上有一个新的T-DNA整合，在3号染色体上有6.55 kb的染色体片段和9号染色体上有29.8 kb的染色体间易位。我们报道了水稻（Oryza sativa）中复杂的农杆菌T-DNA整合与两个染色体间易位相关。T-DNA标记的水稻突变体TC-19携带一个T-DNA拷贝，在纯合条件下表现出雄性不育表型。通过基因组行走分析T-DNA末端与水稻基因组之间的连接，结果表明，T-DNA右侧边缘是3号染色体序列，左侧边缘是9号染色体序列。在3号染色体上进一步行走，检测到3号染色体/ 8号染色体融合。基因组从8号染色体断裂点的另一端行走，发现8号染色体/ 9号染色体融合。我们的研究结果表明，T-DNA以及3号染色体的6.55 kb区域和9号染色体的29.8 kb区域被易位到8号染色体上。对TC-19纯合子突变体进行Southern blot分析发现，3号染色体和9号染色体的原基因序列得到了恢复，但8号染色体Os08g0152500基因第一个内含子的断裂未得到恢复。复杂的T-DNA在8号染色体上的整合导致了男性不育。
A male sterile transgenic rice plant TC-19 harboured a novel T-DNA integration in chromosome 8 with two interchromosomal translocations of 6.55 kb chromosome 3 and 29.8 kb chromosome 9 segments.We report a complex Agrobacterium T-DNA integration in rice (Oryza sativa) associated with two interchromosomal translocations. The T-DNA-tagged rice mutant TC-19, which harboured a single copy of the T-DNA, displayed male sterile phenotype in the homozygous condition. Analysis of the junctions between the T-DNA ends and the rice genome by genome walking showed that the right border is flanked by a chromosome 3 sequence and the left border is flanked by a chromosome 9 sequence. Upon further walking on chromosome 3, a chromosome 3/chromosome 8 fusion was detected. Genome walking from the opposite end of the chromosome 8 break point revealed a chromosome 8/chromosome 9 fusion. Our findings revealed that the T-DNA, together with a 6.55-kb region of chromosome 3 and a 29.8-kb region of chromosome 9, was translocated to chromosome 8. Southern blot analysis of the homozygous TC-19 mutant revealed that the native sequences of chromosome 3 and 9 were restored but the disruption of chromosome 8 in the first intron of the gene Os08g0152500 was not restored. The integration of the complex T-DNA in chromosome 8 caused male sterility.